阿萨伊果（Euterpe oleracea Mart）对MCF-7乳腺癌细胞系诱导的超微结构变化。
Ultrastructural changes induced by açaí (Euterpe oleracea Mart) in MCF-7 breast cancer cell line.
作者信息
da Silva Marcos Antonio Custódio Neto, Soares Camila Simões, Borges Kátia Regina Assunção, Wolff Laís Araujo Souza, Barbosa Maria Do Carmo Lacerda, Nascimento Maria Do Desterro Soares Brandão, Carvalho João Ernesto de
机构信息
Faculty of Medical Science, Post-graduate Program in Internal Medicine, Universidade Estadual de Campi- nas. Rua Tessália Vieira de Camargo, Campinas, Brasil.
Faculty of Pharmaceutical Sciences, Universidade Estadual de Campinas, Campinas, Brazil.
出版信息
Ultrastruct Pathol. 2022 Nov 2;46(6):511-518. doi: 10.1080/01913123.2022.2141404. Epub 2022 Nov 6.
Açaí ( Mart) is an Amazon plant with many biological properties. Previous report of this group evidenced autophagy induction after treatment with açaí seed extract in MCF-7 breast cancer cell lines by acridine orange assay. The aim of this study was to evaluate the ultrastructural changes induced by açaí seed extract in MCF-7 breast cancer cell lines. First, MCF- 7 breast cancer cell line viability was evaluated by MTT assay. Acridine orange assay showed increase in the acidic compartments, suggesting autophagolysosome formation. These cells were treated with 25 μg/ml for 24 h and evaluated by transmission electron microscopy (MET). This analysis showed that açaí seed extract induced autophagy, confirmed by autophagolysosome formation. Furthermore, açaí seed extract increased the number of mitochondria, suggesting the enrollment of reactive oxygen species in autophagy.
阿萨伊（Mart）是一种具有多种生物学特性的亚马逊植物。该研究小组之前的报告通过吖啶橙试验证明，用阿萨伊种子提取物处理MCF - 7乳腺癌细胞系后会诱导自噬。本研究的目的是评估阿萨伊种子提取物在MCF - 7乳腺癌细胞系中诱导的超微结构变化。首先，通过MTT试验评估MCF - 7乳腺癌细胞系的活力。吖啶橙试验显示酸性区室增加，提示自噬溶酶体形成。用25μg/ml的提取物处理这些细胞24小时，然后通过透射电子显微镜（MET）进行评估。该分析表明，阿萨伊种子提取物诱导了自噬，这通过自噬溶酶体的形成得到证实。此外，阿萨伊种子提取物增加了线粒体的数量，提示活性氧参与了自噬过程。
Zotero 插件