Duc Hoang Minh, Zhang Yu, Son Hoang Minh, Huang Hung-Hsin, Masuda Yoshimitsu, Honjoh Ken-Ichi, Miyamoto Takahisa
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Veterinary Public Health, Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Viet Nam.
Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Int J Food Microbiol. 2023 Jan 16;385:109999. doi: 10.1016/j.ijfoodmicro.2022.109999. Epub 2022 Oct 29.
As one major foodborne pathogen, Salmonella can cause serious food poisoning outbreaks worldwide. Bacteriophage therapy is increasingly considered as one of the promising antibacterial agents for the biocontrol of foodborne pathogens. In the current study, a lytic phage STG2 capable of infecting S. enteritidis and S. typhimurium was characterized, and its efficacy in reducing these foodborne pathogens in both planktonic and biofilm forms was evaluated on cabbage and various surfaces. Genomic characterization revealed that phage STG2 was Siphoviridae phage (Epseptimavirus genus) with a dsDNA genome comprising of 114,275 bp and its genome does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing (>2 Log) planktonic cells on cabbage as well as the biofilms formed on cabbage, polystyrene, and stainless steel, suggesting that phage STG2 is capable of simultaneously controlling both S. enteritidis and S. typhimurium contaminations on food and food-related surfaces.
作为一种主要的食源性病原体，沙门氏菌可在全球范围内引发严重的食物中毒疫情。噬菌体疗法日益被视为用于食源性病原体生物防治的一种有前景的抗菌剂。在本研究中，对一种能够感染肠炎沙门氏菌和鼠伤寒沙门氏菌的裂解性噬菌体STG2进行了特性分析，并评估了其在卷心菜和各种表面上减少浮游态和生物膜态食源性病原体的效果。基因组特性分析表明，噬菌体STG2属于长尾噬菌体科噬菌体（埃普西病毒属），其双链DNA基因组由114,275个碱基对组成，且其基因组不包含任何与抗生素抗性、毒素、溶原性或毒力因子相关的基因。此外，噬菌体STG2在减少卷心菜上的浮游细胞（>2个对数）以及在卷心菜、聚苯乙烯和不锈钢上形成的生物膜方面表现出巨大效果，这表明噬菌体STG2能够同时控制食品和食品相关表面上的肠炎沙门氏菌和鼠伤寒沙门氏菌污染。
