Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China.
Jiangsu Key Laboratory of Zoonosis/Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China; Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality of Ministry of Agriculture and Rural Affairs, Yangzhou University, Yangzhou 225009, China.
Vet Microbiol. 2022 Jun;269:109432. doi: 10.1016/j.vetmic.2022.109432. Epub 2022 Apr 14.
As a natural alternative to traditional antimicrobials, phages are being recognised as highly effective control agents for Salmonella and other foodborne bacteria. Due to the high diversity of Salmonella serotypes and the emergence of phage-resistant strains, attempting to isolate more widespread, strictly lytic Salmonella phages is highly warranted. In this study, a lytic phage, LP31, was isolated from poultry faecal samples. Transmission electron microscopy revealed that the phage had a polyhedral head and a retraction-free tail, indicative of the Siphoviridae family. Adsorption rate experiments showed that LP31 required the participation of lipopolysaccharides, but not flagella, during phage adsorption. Host profile identification showed that LP31 could lyse most Salmonella Enteritidis (S. Enteritidis) (96.15%, N = 104) and Salmonella Pullorum (S. Pullorum) (96.67%, N = 60). Initial applications found that LP31 reduced the concentration of static S. Enteritidis on metal surfaces (0.951 log cfu/ml) and in the faeces of chicks (2.14 log cfu/g). Notably, LP31 could almost completely remove biofilms formed by S. Enteritidis and S. Pullorum in 1 h. These findings suggest that LP31 has a good prevention and control effect against biofilms and planktonic antibiotic-resistant Salmonella, and is therefore a potentially promising biocontrol agent for controlling the spread of Salmonella in the poultry and food processing industries.
作为传统抗菌剂的天然替代品，噬菌体被认为是控制沙门氏菌和其他食源性细菌的高效控制剂。由于沙门氏菌血清型的高度多样性和噬菌体抗性菌株的出现，尝试分离更广泛、严格裂解的沙门氏菌噬菌体是非常有必要的。在这项研究中，从禽粪样中分离出一种裂解噬菌体 LP31。透射电子显微镜显示，该噬菌体具有多面体形头部和无收缩的尾部，表明其属于肌尾噬菌体科。吸附率实验表明，LP31 在噬菌体吸附过程中需要脂多糖的参与，但不需要鞭毛。宿主谱鉴定表明，LP31 可以裂解大多数肠炎沙门氏菌（S. Enteritidis）（96.15%，N=104）和鸡白痢沙门氏菌（S. Pullorum）（96.67%，N=60）。初步应用发现，LP31 可降低金属表面（0.951 log cfu/ml）和雏鸡粪便中（2.14 log cfu/g）静止肠炎沙门氏菌的浓度。值得注意的是，LP31 可以在 1 小时内几乎完全去除肠炎沙门氏菌和鸡白痢沙门氏菌形成的生物膜。这些发现表明，LP31 对生物膜和浮游耐药性沙门氏菌具有良好的预防和控制作用，因此是控制沙门氏菌在禽畜和食品加工业中传播的一种有潜力的生物防治剂。
