Department of Pharmaceutical Biotechnology, Faculty of Pharmacy & Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, PO Box 14155-6451, Iran.
Department of Food and Drug Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Vet Res Commun. 2023 Sep;47(3):1493-1503. doi: 10.1007/s11259-023-10105-1. Epub 2023 Apr 25.
Salmonella enteritidis is one of the most important foodborne pathogens that cause numerous outbreaks worldwide. Some strains of Salmonella have become progressively resistant to antibiotics, so they could represent a critical threat to public health and have led to the use of alternative therapeutic approaches like phage therapy. In this study, a lytic phage, vB_SenS_TUMS_E4 (E4), was isolated from poultry effluent and characterized to evaluate its potential and efficacy for bio-controlling S. enteritidis in foods. Transmission electron microscopy revealed that E4 has a siphovirus morphotype, with an isometric head and non-contractile tail. Determining the host range showed that this phage can effectively infect different motile as well as non-motile Salmonella enterica serovars. The biological characteristics of E4 showed that it has a short latent period of about 15 min and a large burst size of 287 PFU/cell, and is also significantly stable in a broad range of pHs and temperatures. The E4 whole genome contains 43,018 bp and encodes 60 coding sequences (CDSs) but no tRNA genes. Bioinformatics analysis revealed that the genome of E4 lacks any genes related to lysogeny behavior, antibiotic resistance, toxins, or virulence factors. The efficacy of phage E4 as a bio-control agent was assessed in various foodstuffs inoculated with S. enteritidis at 4°C and 25°C, and the resulting data indicated that it could eradicate S. enteritidis after a very short time of 15 min. The findings of the present study showed that E4 is a hopeful candidate as a bio-control agent against S. enteritidis and has the potential to be used in various foodstuffs.
肠炎沙门氏菌是引起全球众多暴发的最重要食源性病原体之一。一些肠炎沙门氏菌菌株对抗生素的耐药性逐渐增强，因此它们可能对公众健康构成严重威胁，并导致人们转而使用噬菌体治疗等替代治疗方法。在本研究中，从禽畜污水中分离出一种裂解噬菌体 vB_SenS_TUMS_E4（E4），并对其进行了特性描述，以评估其在食品中控制肠炎沙门氏菌的潜力和效果。透射电子显微镜显示，E4 具有一种长尾噬菌体科的形态，具有等径头部和非收缩性尾部。宿主范围的确定表明，这种噬菌体可以有效地感染不同的运动性和非运动性肠炎沙门氏菌血清型。E4 的生物学特性表明，其潜伏期约为 15 分钟，爆发量较大，为 287PFU/细胞，在较宽的 pH 和温度范围内也非常稳定。E4 的全基因组包含 43018bp，编码 60 个编码序列（CDS），但没有 tRNA 基因。生物信息学分析表明，E4 的基因组缺乏任何与溶原行为、抗生素耐药性、毒素或毒力因子相关的基因。噬菌体 E4 作为生物控制剂的功效在接种肠炎沙门氏菌的各种食品中进行了评估，结果表明，在 4°C 和 25°C 下，E4 可在非常短的 15 分钟内根除肠炎沙门氏菌。本研究的结果表明，E4 是一种有希望的肠炎沙门氏菌生物控制剂候选物，具有在各种食品中应用的潜力。
