Lu Yapeng, Zhu Xiaopeng, Hu Chao, Li Peng, Zhao Meihui, Lu Jinfeng, Xia Guanghua
Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Key Laboratory of Seafood Processing of Haikou, College of Food Science and Engineering, Hainan University, Hainan 570228, China.
Hainan Engineering Research Center of Aquatic Resources Efficient Utilization in South China Sea, Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Key Laboratory of Seafood Processing of Haikou, College of Food Science and Engineering, Hainan University, Hainan 570228, China; Collaborative Innovation Center of Provincial and Ministerial Co-Construction for Marine Food Deep Processing, Dalian Polytechnic University, Dalian 116034, China.
Int J Biol Macromol. 2022 Dec 31;223(Pt A):36-48. doi: 10.1016/j.ijbiomac.2022.10.255. Epub 2022 Nov 3.
Microbial infections and the slow regression of inflammation are major impediments to wound healing. Herein, a tilapia fish skin gelatin-fucose gum-tannic acid (Gel&Fuc-TA) hydrogel wound dressing (Gel&Fuc-TA) was designed to promote wound healing by mixing and reacting tannic acid (TA) with tilapia fish skin gelatin (Gel) and fucoidan (Fuc). Gel&Fuc-TA hydrogel has a good network structure as well as swelling and release properties, and shows excellent antibacterial, antioxidant, cell compatibility, and hemostatic properties. Gel&Fuc-TA hydrogel can promote the expression of vascular endothelial growth factor (VEGF), platelet endothelial cell adhesion molecule-1 (CD-31), and alpha-smooth muscle actin (α-SMA), enhance collagen deposition, and accelerate wound repair. Gel&Fuc-TA hydrogel can change the wound microbiome, reduce wound microbiome colonization, and decrease the expression of microbiome-related proinflammatory factors, such as lipopolysaccharide (LPS), Toll-like receptor 2 (TLR2), and Toll-like receptor 4 (TLR4). Gel&Fuc-TA hydrogel effectively regulates the conversion of wound macrophages to the M2 (anti-inflammatory phenotype) phenotype, decreases the expression of interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α), and increases the expression of arginase-1 (Arg-1), interleukin-10 (IL-10) and transforming growth factor-beta (TGF-β), thereby reducing the inflammatory response. In summary, Gel&Fuc-TA hydrogel prepared using a rational green cross-linking reaction can effectively accelerate wound healing.
微生物感染以及炎症的缓慢消退是伤口愈合的主要障碍。在此，通过将单宁酸（TA）与罗非鱼鱼皮明胶（Gel）和岩藻依聚糖（Fuc）混合并反应，设计了一种罗非鱼鱼皮明胶 - 岩藻依聚糖 - 单宁酸（Gel&Fuc - TA）水凝胶伤口敷料，以促进伤口愈合。Gel&Fuc - TA水凝胶具有良好的网络结构以及溶胀和释放特性，并表现出优异的抗菌、抗氧化、细胞相容性和止血性能。Gel&Fuc - TA水凝胶可促进血管内皮生长因子（VEGF）、血小板内皮细胞黏附分子 - 1（CD - 31）和α - 平滑肌肌动蛋白（α - SMA）的表达，增强胶原蛋白沉积，并加速伤口修复。Gel&Fuc - TA水凝胶可改变伤口微生物群，减少伤口微生物群定植，并降低微生物群相关促炎因子如脂多糖（LPS）、Toll样受体2（TLR2）和Toll样受体4（TLR4）的表达。Gel&Fuc - TA水凝胶有效地调节伤口巨噬细胞向M2（抗炎表型）表型的转化，降低白细胞介素 - 6（IL - 6）、白细胞介素 - 1β（IL - 1β）和肿瘤坏死因子 - α（TNF - α）的表达，并增加精氨酸酶 - 1（Arg - 1）、白细胞介素 - 10（IL - 10）和转化生长因子 - β（TGF - β）的表达，从而减轻炎症反应。总之，通过合理的绿色交联反应制备的Gel&Fuc - TA水凝胶可有效加速伤口愈合。
