来自……的嗜盐漆酶对蒽的生物去除与解毒作用
Bioremoval and Detoxification of Anthracene by a Halophilic Laccase from .
作者信息
Rahimi Elaheh, Rezaei Shahla, Mohamadnia Sonia, Valizadeh Shiler, Tavakoli Omid, Faramarzi Mohammad Ali
机构信息
School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 14176, Islamic Republic of Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155-6451, Tehran 1417614411, Islamic Republic of Iran.
出版信息
Iran J Biotechnol. 2022 Apr 1;20(2):e3058. doi: 10.30498/ijb.2022.287500.3058. eCollection 2022 Apr.
BACKGROUND
Polycyclic Aromatic Hydrocarbons (PAHs) as resistant compounds in the environment has received much attention in recent years due to their adverse effects on ecological health. Among the various methods studying the removal of PAHs, enzyme biotechnology is one of the most effective and appropriate method.
OBJECTIVES
In the present study, a halophilic laccase was used for bioremoval of anthracene in the presence of 1-Hydroxybenzotriazole (HBT).
MATERIALS AND METHODS
Halophilic laccase from was tested for anthracene degradation. Residual concentration of anthracene at various concentrations of NaCl (0‒4 M), incubation time, pH, solvent, and surfactants in the enzymatic reaction mixtures was determined by HPLC.
RESULTS
The maximum removal of substrate was achieved after 72 h at 40 °C, pH 8, and NaCl concentration 1.5 M. Besides, the addition of 1% (v/v) ionic and non-ionic surfactants and 25% (v/v) of various organic solvents increased removal efficiency. The kinetic parameters K and V of the laccase for removing of anthracene were 0.114 μM and 0.546 μmoL. h. mg, respectively.
CONCLUSIONS
Laccase showed the maximum removal efficiency of anthracene in the presence of 1-Hydroxybenzotriazole (HBT).
背景
多环芳烃（PAHs）作为环境中的抗性化合物，近年来因其对生态健康的不利影响而备受关注。在研究PAHs去除的各种方法中，酶生物技术是最有效且合适的方法之一。
目的
在本研究中，使用嗜盐漆酶在1-羟基苯并三唑（HBT）存在的情况下对蒽进行生物去除。
材料与方法
测试了来自[具体来源未给出]的嗜盐漆酶对蒽的降解作用。通过高效液相色谱法测定酶促反应混合物中不同NaCl浓度（0‒4 M）、孵育时间、pH、溶剂和表面活性剂条件下蒽的残留浓度。
结果
在40℃、pH 8和NaCl浓度1.5 M的条件下，72小时后底物的去除率达到最高。此外，添加1%（v/v）的离子和非离子表面活性剂以及25%（v/v）的各种有机溶剂可提高去除效率。漆酶去除蒽的动力学参数K和V分别为0.114 μM和0.546 μmoL·h·mg。
结论
在1-羟基苯并三唑（HBT）存在的情况下，漆酶对蒽的去除效率最高。
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