Wang Jian, Yang Tong, Li Zhengjia, Zhou Kecen, Xiao Bo, Yu Peng
School of Materials Science and Engineering, Xiangtan University Xiangtan 411105 China
RSC Adv. 2022 Oct 26;12(47):30457-30465. doi: 10.1039/d2ra03892g. eCollection 2022 Oct 24.
Excess exposure to a high environmental level of nickel can cause serious harm to human health. Therefore, it is particularly necessary to develop a low-cost, fast and sensitive method for nickel assay. In this paper, novel counting-based microfluidic paper-based devices (μPADs) were prepared by hand drawing and yellow oily double-sided adhesive tape. The dissolved adhesive tape was used for the first time to make the hydrophobic "ink". The marker filled with the "ink" drew the desirable layout on paper followed by a drying process. The μPADs were constituted of one circular sample introduction zone (diameter 4.5 mm) and four circular detection zones (diameter 3 mm). The adjoining detection zones were connected by a strip channel (1.2 mm × 2 mm). The fabrication conditions were optimized and the barriers created with the marker revealed good reproducibility. The analytical performance of the developed devices was investigated for nickel assay. The Ni standard was added to the sample introduction zone, and subsequently moved into the detection zones containing dimethylglyoxime (DMG), where it reacted with Ni and formed a reddish pink Ni-DMG complex. Through counting the number of colored dots, the developed μPADs realized the semi-quantitative analysis of nickel. More importantly, the fabrication of the developed counting-based μPADs only used a marker and adhesive tape, possessing the advantages of instrument-free simplicity and low cost. Our method has great potential for enabling μPADs to be easily implemented in laboratories or research centers with limited resources.
过度暴露于高环境水平的镍会对人体健康造成严重危害。因此，开发一种低成本、快速且灵敏的镍检测方法尤为必要。本文通过手绘和黄色油性双面胶带制备了新型基于计数的微流控纸基器件（μPADs）。首次使用溶解后的胶带制作疏水“墨水”。用填充有这种“墨水”的记号笔在纸上绘制出理想的布局，随后进行干燥处理。μPADs由一个圆形样品引入区（直径4.5毫米）和四个圆形检测区（直径3毫米）组成。相邻的检测区通过一条条形通道（1.2毫米×2毫米）相连。对制作条件进行了优化，用记号笔绘制的屏障显示出良好的重现性。对所开发器件用于镍检测的分析性能进行了研究。将镍标准品添加到样品引入区，随后其进入含有丁二酮肟（DMG）的检测区，在那里它与镍反应形成淡红色的镍 - 丁二酮肟络合物。通过计算有色斑点的数量，所开发的μPADs实现了对镍的半定量分析。更重要的是，所开发的基于计数的μPADs的制作仅使用了一支记号笔和胶带，具有无需仪器的简便性和低成本的优点。我们的方法具有巨大潜力，能够使μPADs在资源有限的实验室或研究中心轻松实现。
