Key Laboratory of Low-Dimensional Materials and Application Technologies (Ministry of Education), School of Materials Science and Engineering, Xiangtan University, Xiangtan 411105, China.
Sensors (Basel). 2019 Sep 21;19(19):4082. doi: 10.3390/s19194082.
A simple, low-cost technique has been developed for the rapid fabrication of single-layered paper-based microfluidic devices (μPADs). This technique, for the first time, made use of the deposition of patterned adhesive tape into the filter paper to construct hydrophobic barriers, with the help of toluene. Unlike other reported multi-layered μPADs that merely made use of adhesive tape as a separate layer for sealing or fluid flow controlling, the patterned adhesive tape was simultaneously dissolved and penetrated into the filter paper, which resulted in the successful transfer of the pattern from the tape to the filter paper. To demonstrate the effectiveness of this approach, nitrite and glucose were individually measured; detection limits as low as 0.015 ± 0.004 mM and 0.022 ± 0.006 mM were reported for nitrite and glucose, respectively. Multiplexed analysis of both analytes was also carried out with respective detection limits of 0.048 ± 0.005 mM and 0.025 ± 0.006 mM for nitrite and glucose. The application of the method was demonstrated by measuring nitrite and glucose in spiked artificial urine samples and satisfied recovery results were obtained.
一种简单、低成本的技术已经被开发出来，用于快速制作单层纸基微流控装置（μPADs）。该技术首次利用甲苯将图案化的胶带沉积到滤纸上，构建疏油屏障。与其他报道的多层 μPADs 不同，这些多层 μPADs仅仅将胶带用作单独的层来进行密封或控制流体流动，而图案化的胶带则同时溶解并渗透到滤纸上，从而成功地将图案从胶带转移到滤纸上。为了证明这种方法的有效性，分别对亚硝酸盐和葡萄糖进行了测量；报告的亚硝酸盐和葡萄糖的检测限分别低至 0.015 ± 0.004 mM 和 0.022 ± 0.006 mM。还对两种分析物进行了多重分析，亚硝酸盐和葡萄糖的检测限分别为 0.048 ± 0.005 mM 和 0.025 ± 0.006 mM。该方法的应用通过测量加标人工尿液样品中的亚硝酸盐和葡萄糖得到了验证，并获得了满意的回收率结果。
