Ren Jing, Li Xiaodan, Zhang Weitao, Li Zhuofan, Wang Quan, Li Shuna, Wang Shuxiang, Li Hongya
College of Life Sciences, Hebei Agricultural University, Baoding, China.
Hebei Animal Husbandry General Station, Shijiazhuang, Hebei, China.
Front Microbiol. 2022 Oct 21;13:1031853. doi: 10.3389/fmicb.2022.1031853. eCollection 2022.
Pulp and paper mill effluent is rich in recalcitrant and toxic pollutants compounds and causes pollution. To find an efficient biocatalyst for the treatment of effluent, a dye-decolorizing peroxidase from MN-13, which is capable of degrading lignin, was used for the bioremediation of paper and pulp mill effluent. The dye-decolorizing peroxidase from (BaDyP) exhibited high-redox potential to 2, 2'-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) ammonium salt (ABTS), veratryl alcohol, Mn, reactive blue 19, reactive black 5 and lignin dimer guaiacylglycerol-beta-guaiacyl ether (GGE). When GGE was used as substrate, BaDyP broke β-O-4 bond of GGE and then oxidize Cα to generate vanillin. The values for ABTS and veratryl alcohol were 2.19 mm and 0.07 mm, respectively. The for ABTS and veratryl alcohol were 1.8 mm/min and 14.12 mm/min, respectively. The BaDyP-mediated treatment of pulp and paper mill effluent led to significant reduction of chemical oxygen demand (COD) and color. When 5% (v/v) of effluent was treated with BaDyP for 12 h at 30°C and pH 2, the removal of COD, color, and lignin was achieved at 82.7, 80.2, and 78.20%, respectively. In detoxification assay, the seeds of grown in treated effluent showed a significant increase in germination rate from 66.7% (untreated effluent) to 90%, and in radicle length from 0.68 cm (untreated effluent) to 1.26 cm, respectively. In the meanwhile, the inhibition of and by the treated effluent reduced significantly as compared to untreated effluent, indicating high detoxification performance of BaDyP for the treatment of pulp and paper mill effluent. The findings suggest that BaDyP is a potential catalyst for bioremediation of pulp and paper mill effluent, as it is effective in substantial lowering of pollutants load as well as reduces COD, color, and toxicity of effluent.
制浆造纸厂废水富含难降解和有毒污染物化合物，会造成污染。为了找到一种处理废水的高效生物催化剂，来自MN - 13的一种能够降解木质素的染料脱色过氧化物酶被用于制浆造纸厂废水的生物修复。来自（BaDyP）的染料脱色过氧化物酶对2, 2'-偶氮双（3 - 乙基苯并噻唑啉 - 6 - 磺酸）铵盐（ABTS）、藜芦醇、锰、活性蓝19、活性黑5和木质素二聚体愈创木基甘油 - β - 愈创木基醚（GGE）表现出高氧化还原电位。当GGE用作底物时，BaDyP断裂GGE的β - O - 4键，然后氧化Cα生成香草醛。ABTS和藜芦醇的值分别为2.19 mm和0.07 mm。ABTS和藜芦醇的分别为1.8 mm/分钟和14.12 mm/分钟。BaDyP介导的制浆造纸厂废水处理导致化学需氧量（COD）和颜色显著降低。当5%（v/v）的废水在30°C和pH 2下用BaDyP处理12小时时，COD、颜色和木质素的去除率分别达到82.7%、80.2%和78.20%。在解毒试验中，在处理后的废水中生长的种子发芽率从66.7%（未处理废水）显著提高到90%，胚根长度从0.68 cm（未处理废水）增加到1.26 cm。同时，与未处理废水相比，处理后的废水对和的抑制作用显著降低，表明BaDyP对制浆造纸厂废水的处理具有高解毒性能。研究结果表明，BaDyP是制浆造纸厂废水生物修复的潜在催化剂，因为它有效地大幅降低了污染物负荷以及降低了废水的COD、颜色和毒性。
