College of Life Science, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Biotechnol Lett. 2019 Sep;41(8-9):1015-1021. doi: 10.1007/s10529-019-02696-0. Epub 2019 May 27.
The dye-decolorizing peroxidase from Bacillus amyloliquefaciens, BaDyP, was identified to be an efficient catalyst for the degradation of phenolic β-ether lignin model dimer guaiacylglycerol-β-guaiacyl ether (GGE) and dyes.
Efeb gene encoding BaDyP from B. amyloliquefaciens MN-13 consisted of 1257 bp and the open reading frame encoded 418 amino acids. The efeb gene was expressed in Escherichia coli BL21 and a recombinant BaDyP of 50 kDa was achieved. The BaDyP exhibited activity in oxidizing GGE and decolorizing azo and triphenylmethane dyes. At pH 4.5 and 30 °C the BaDyP not only completely degraded GGE by the cleavage of β-O-4 ether bond and Cα-C bond, and Cα oxidation without any oxidative mediator, but also decolorized four synthetic dyes, including congo red, bromine cresol green, eriochrome black T and crystal violet. This was achieved with decolorization rates of 65.7%, 70.62%, 80.06% and 62.09%, respectively, after 72 h of incubation.
BaDyP was identified as a bacteria peroxidase with great potential for the degradation of lignin and bioremediation of dye-contamination.
从解淀粉芽孢杆菌中鉴定出的染料脱色过氧化物酶 BaDyP 是一种有效催化酚型 β-醚型木质素模型二聚体愈创木基甘油-β-愈创木醚（GGE）和染料降解的催化剂。
来源于 B. amyloliquefaciens MN-13 的 efeb 基因包含 1257bp，开放阅读框编码 418 个氨基酸。efeb 基因在大肠杆菌 BL21 中表达，得到 50kDa 的重组 BaDyP。BaDyP 表现出氧化 GGE 和脱色偶氮和三苯甲烷染料的活性。在 pH4.5 和 30°C 下，BaDyP 不仅可以在没有任何氧化介体的情况下通过β-O-4 醚键和 Cα-C 键的裂解以及 Cα 氧化完全降解 GGE，还可以脱色四种合成染料，包括刚果红、溴甲酚绿、铬黑 T 和结晶紫。经过 72 小时孵育，分别达到 65.7%、70.62%、80.06%和 62.09%的脱色率。
BaDyP 被鉴定为一种具有巨大潜力的细菌过氧化物酶，可用于木质素的降解和染料污染的生物修复。
