Ye Congxiu, Zhang Yunqing, Su Zhen, Wu Shuxia, Li Yuxia, Yi Jinling, Lai Wei, Chen Jian, Zheng Yue
Department of Dermato-venereology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
AIE Bioscience (Guangdong) Co., LTD., Torch Development Zone, Zhongshan, Guangdong, China.
Front Bioeng Biotechnol. 2022 Oct 21;10:1053679. doi: 10.3389/fbioe.2022.1053679. eCollection 2022.
Recent studies have reported that the incidence of sensitive skin is increasing. Skin sensitivity and skin barrier functions were related to many skin diseases including atopic dermatitis, psoriasis, rosacea, and so on. Mesenchymal stem cell (MSC)-derived exosomes (hMSC) might be considered as a new effective therapeutic scheme. This study aims to investigate the safety and efficacy of hMSC exosomes as a novel topical treatment for sensitive skin. Exosomes were extracted from primary hMSC ultracentrifugation method. The morphology of hMSC exosomes was studied transmission electron microscope. Expression of exosome specific surface marker was detected Western blot. 22 subjects (female, aged 18-55) diagnosed with sensitive skin were enrolled. Follow-up was conducted before, 7-day, 14-day, and 28-day after hMSC exosomes use. Transepidermal water loss (TEWL), surface hydration, sebum secretion, and Lab* value were simultaneously tested at the same time point in an environment-controlled room. Under transmission electron microscopy, the extracted hMSC exosomes were circular or elliptical with intact membrane structure, and their diameters ranged mainly from 40 to 80 nm. Western blot showed that the expression of markers CD63, CD9, and Tsg101 was positive. Brownian motion based nanoparticle trajectory analysis (NTA) showed that the main peak of particle size distribution occurred around 96 nm, the average particle size was 122 nm, and the main peak accounted for 96.7%. All this conformed to the biological characteristics of exosomes standardized by the International Society for Extracellular Vesicles. In the clinical trial, scores of objective symptoms including roughness, scales, erythema, and subjective symptoms including tension, burning, or itching, were improved after 7-, 14-, and 28- day using hMSC-exosomes. TEWL, hydration, sebum, pH, and a* values were tended to return to the level of healthy skin. The hMSC-exosomes, with the advantages of biocompatibility and biodegradability, could improve clinical symptoms and eruptions in sensitive skin patients, and might be as an MSC cell-free novel therapy in sensitive skin-related disease treatment.
最近的研究报告称，敏感性皮肤的发病率正在上升。皮肤敏感性和皮肤屏障功能与许多皮肤疾病有关，包括特应性皮炎、银屑病、玫瑰痤疮等。间充质干细胞（MSC）衍生的外泌体（hMSC）可能被视为一种新的有效治疗方案。本研究旨在探讨hMSC外泌体作为一种新型局部治疗敏感性皮肤的安全性和有效性。外泌体通过超速离心法从原代hMSC中提取。通过透射电子显微镜研究hMSC外泌体的形态。通过蛋白质印迹法检测外泌体特异性表面标志物的表达。招募了22名被诊断为敏感性皮肤的受试者（女性，年龄18 - 55岁）。在使用hMSC外泌体之前、7天、14天和28天后进行随访。在环境控制的房间内的同一时间点同时测试经表皮水分流失（TEWL）、表面水合作用、皮脂分泌和Lab值。在透射电子显微镜下，提取的hMSC外泌体呈圆形或椭圆形，膜结构完整，其直径主要在40至80纳米之间。蛋白质印迹法显示标志物CD63、CD9和Tsg101的表达呈阳性。基于布朗运动轨迹分析的纳米颗粒跟踪分析（NTA）显示，粒径分布的主峰出现在约96纳米处，平均粒径为122纳米，主峰占96.7%。所有这些均符合国际细胞外囊泡协会标准化的外泌体生物学特征。在临床试验中，使用hMSC - 外泌体7天、14天和28天后，包括粗糙度、鳞屑、红斑等客观症状评分以及包括紧绷感、烧灼感或瘙痒感等主观症状均得到改善。TEWL、水合作用、皮脂、pH值和a值趋于恢复到健康皮肤水平。hMSC - 外泌体具有生物相容性和可生物降解性的优点，可改善敏感性皮肤患者的临床症状和皮疹，可能作为一种无MSC细胞的新型疗法用于治疗与敏感性皮肤相关的疾病。
