不列颠哥伦比亚省出现侵袭性阴性血清型e的克隆菌株。
Emergence of a clone of invasive -negative serotype e in British Columbia.
作者信息
Whyte Kathleen E, Hoang Linda, Sekirov Inna, Shuel Michelle L, Hoang William, Tsang Raymond Sw
机构信息
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
BC Public Health Microbiology and Reference Laboratory, Vancouver, British Columbia, Canada.
出版信息
J Assoc Med Microbiol Infect Dis Can. 2020 Mar 4;5(1):29-34. doi: 10.3138/jammi.2019-0015. eCollection 2020 Mar.
BACKGROUND
Introduction of the serotype b (Hib) conjugate vaccine has changed the epidemiology of invasive disease, with most infections now caused by non-typeable (non-encapsulated) and non-Hib encapsulated strains.
METHODS
We describe nine invasive serotype e (Hie) from British Columbia that were determined to have complete deletion of their fucose operon genes. These nine isolates were recovered from blood cultures of three female and six male patients during 2011-2018, with eight recovered in the past 4 years.
RESULTS
All nine isolates were biotype IV, with eight showing identical pulsed field gel electrophoresis (PFGE) profiles, whereas the ninth showed 95% similarity. PFGE analysis also showed these fucose operon-negative Hie to be most (94%) similar to the multi-locus sequence type (ST)-18, the most common ST among Hie in British Columbia. These nine fucose operon-negative Hie represented 27.3% of the 33 invasive Hie isolated in British Columbia from 2010 to 2018.
CONCLUSION
Deletion of the fucose operon did not appear to impact the transmission ability of these strains or their ability to cause invasive disease.
背景
b型流感嗜血杆菌（Hib）结合疫苗的引入改变了侵袭性疾病的流行病学，目前大多数感染由不可分型（非包膜）和非Hib包膜菌株引起。
方法
我们描述了来自不列颠哥伦比亚省的9株侵袭性e型流感嗜血杆菌（Hie），这些菌株被确定其岩藻糖操纵子基因完全缺失。这9株分离株是在2011年至2018年期间从3名女性和6名男性患者的血培养物中分离得到的，其中8株是在过去4年中分离得到的。
结果
所有9株分离株均为生物IV型，其中8株显示相同的脉冲场凝胶电泳（PFGE）图谱，而第9株显示95%的相似性。PFGE分析还显示，这些岩藻糖操纵子阴性的Hie与多位点序列类型（ST）-18最为相似（94%），ST-18是不列颠哥伦比亚省Hie中最常见的ST。这9株岩藻糖操纵子阴性的Hie占2010年至2018年在不列颠哥伦比亚省分离的33株侵袭性Hie的27.3%。
结论
岩藻糖操纵子的缺失似乎并未影响这些菌株的传播能力或其引起侵袭性疾病的能力。
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