Lafzi Ardeshir, Saravi Najmeh Sadat Valed, Amid Reza, Kadkhodazadeh Mahdi, Shojaei Narges
Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Periodontics, School of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.
J Indian Soc Periodontol. 2022 Sep-Oct;26(5):440-445. doi: 10.4103/jisp.jisp_739_20. Epub 2022 Sep 1.
This study aimed to compare the biological reactions of dental pulp stem cells (DPSCs) cultured on new xenograft bone substitutes derived from camel and bovine bones.
DPSCs were cultured and placed on different xenograft materials including Bone Plus (bovine), Camel Bone, and demineralized bovine bone matrix. The viability and proliferation of cells were evaluated by the methyl thiazolyl tetrazolium assay after 24, 48, and 72 h. The alkaline phosphatase (ALP) test and Alizarin red staining were performed at 7 and 21 days to assess the osteoblastic differentiation of cells. Osteocalcin (OCN) gene expression was evaluated qualitatively at 3-, 7- and 14-days using polymerase chain reaction (PCR). Semi-quantitative PCR was also performed using ImageJ software. Data were analyzed by ANOVA and Tukey's honestly significant difference test.
The cell proliferation rate was significantly different among the three xenograft bone substitutes at 24-, 48- and 72 h ( < 0.05). The ALP activity of DPSCs in all three xenograft bone substitute groups was greater than that in the control group ( < 0.05). Alizarin red staining showed no significant difference in the formation of calcified nodules among the groups. Qualitative and semi-quantitative PCR displayed that the expression of OCN gene in the Camel Bone and Bone Plus groups was higher than that in the demineralized bovine bone matrix group.
The Camel Bone xenograft caused a high proliferation rate and optimal osteogenic differentiation of DPSCs qualitatively and semi-quantitatively . Further studies are required on this xenograft bone substitute.
本研究旨在比较在源自骆驼和牛骨的新型异种移植骨替代物上培养的牙髓干细胞（DPSCs）的生物学反应。
培养DPSCs并将其置于不同的异种移植材料上，包括骨加（牛）、骆驼骨和脱矿牛骨基质。在24、48和72小时后，通过甲基噻唑基四氮唑法评估细胞的活力和增殖。在第7天和第21天进行碱性磷酸酶（ALP）测试和茜素红染色，以评估细胞的成骨分化。在第3、7和14天使用聚合酶链反应（PCR）定性评估骨钙素（OCN）基因表达。还使用ImageJ软件进行半定量PCR。数据通过方差分析和Tukey's真实显著性差异检验进行分析。
在24、48和72小时时，三种异种移植骨替代物之间的细胞增殖率存在显著差异（<0.05）。所有三个异种移植骨替代物组中DPSCs的ALP活性均高于对照组（<0.05）。茜素红染色显示各组之间钙化结节的形成无显著差异。定性和半定量PCR显示，骆驼骨组和骨加组中OCN基因的表达高于脱矿牛骨基质组。
骆驼骨异种移植在定性和半定量方面导致DPSCs的高增殖率和最佳成骨分化。需要对这种异种移植骨替代物进行进一步研究。
