Tong Xin, Ga Lu, Eerdun Chaolu, Zhao Ruiguo, Ai Jun
College of Chemistry and Enviromental Science, Inner Mongolia Key Laboratory of Environmental Chemistry, Inner Mongolia Normal University, 81 Zhaowudalu, Hohhot 010022, People's Republic of China.
College of Pharmacy, Inner Mongolia Medical University, Jinchuankaifaqu, Hohhot 010110, People's Republic of China.
ACS Omega. 2022 Oct 20;7(43):39224-39233. doi: 10.1021/acsomega.2c05243. eCollection 2022 Nov 1.
Based on the reactions of different sequences with single ions K, Na, NH , double ions high + low, low + high, and triple ions with different addition orders, the best stable ion combinations of 12 quadruplexes with different DNA sequences were reported. From the fluorescence spectrum, except for HT-V15 and PW17 and AS1411 and HT-V18, the structural stability of G-quadrangle formed basically follows a certain rule. In terms of this experiment, according to circular dichroism, the antiparallel quadrupole structure has the largest proportion among quadrupole structures, and 12 optimal DNA addition schemes and sequences have been obtained through exploration. It is worth mentioning that, on the whole, the best addition scheme of AS1411 and HT-V18 is a three-ion scheme, which provides an effective reference for similar experiments in the future.
基于不同序列与单离子K、Na、NH ，双离子高+低、低+高以及不同添加顺序的三离子的反应，报道了12种不同DNA序列的四链体的最佳稳定离子组合。从荧光光谱来看，除了HT-V15和PW17以及AS1411和HT-V18外，形成的G-四边形的结构稳定性基本遵循一定规律。就本实验而言，根据圆二色性，反平行四极结构在四极结构中所占比例最大，并且通过探索获得了12种最佳DNA添加方案和序列。值得一提的是，总体而言，AS1411和HT-V18的最佳添加方案是三离子方案，这为未来类似实验提供了有效参考。
