Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.
Int J Mol Sci. 2021 May 11;22(10):5088. doi: 10.3390/ijms22105088.
Seed germination is a key step in the new life cycle of plants. In agriculture, we regard the rapid and consistent process of seed germination as one of the necessary conditions to measure the high quality and yield of crops. ENO2 is a key enzyme in glycolysis, which also plays an important role in plant growth and abiotic stress responses. In our study, we found that the time of seed germination in mutation () was earlier than that of wild type (WT) in . Previous studies have shown that microRNAs (miRNAs) were vital in seed germination. After deep sequencing of small RNA, we found 590 differentially expressed miRNAs in total, of which 87 were significantly differentially expressed miRNAs. By predicting the target genes of miRNAs and analyzing the GO annotation, we have counted 18 genes related to seed germination, including family, , , , , , , and . MiR9736-z, miR5059-z, ath-miR167a-5p, ath-miR167b, ath-miR5665, ath-miR866-3p, miR10186-z, miR8165-z, ath-miR857, ath-miR399b, ath-miR399c-3p, miR399-y, miR163-z, ath-miR393a-5p, and ath-miR393b-5p are the key miRNAs regulating seed germination-related genes. Through KEGG enrichment analysis, we found that phytohormone signal transduction pathways were significantly enriched, and these miRNAs mentioned above also participate in the regulation of the genes in plant hormone signal transduction pathways, thus affecting the synthesis of plant hormones and further affecting the process of seed germination. This study laid the foundation for further exploration of the regulation for seed germination.
种子萌发是植物新生活周期的关键步骤。在农业中，我们将种子迅速而一致的萌发过程视为衡量作物高质量和高产量的必要条件之一。ENO2 是糖酵解中的关键酶，在植物生长和非生物胁迫响应中也起着重要作用。在我们的研究中，我们发现 在 中的种子萌发时间早于野生型（WT）。先前的研究表明，microRNAs（miRNAs）在种子萌发中至关重要。对小 RNA 进行深度测序后，我们总共发现了 590 个差异表达的 miRNAs，其中 87 个是显著差异表达的 miRNAs。通过预测 miRNA 的靶基因并分析 GO 注释，我们统计了与种子萌发相关的 18 个基因，包括 家族、、、、、、和 。MiR9736-z、miR5059-z、ath-miR167a-5p、ath-miR167b、ath-miR5665、ath-miR866-3p、miR10186-z、miR8165-z、ath-miR857、ath-miR399b、ath-miR399c-3p、miR399-y、miR163-z、ath-miR393a-5p 和 ath-miR393b-5p 是调节种子萌发相关基因的关键 miRNAs。通过 KEGG 富集分析，我们发现植物激素信号转导途径显著富集，上述 miRNAs 也参与了植物激素信号转导途径中基因的调控，从而影响植物激素的合成，进一步影响种子萌发过程。本研究为进一步探索 对种子萌发的调控奠定了基础。
