Eye Institute of Shandong First Medical University, Qingdao Eye Hospital of Shandong First Medical University, State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, School of Ophthalmology, Shandong First Medical University, Qingdao 266071, China; Department of Ophthalmology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, China.
Department of Sports Medicine, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
STAR Protoc. 2022 Oct 29;3(4):101801. doi: 10.1016/j.xpro.2022.101801. eCollection 2022 Dec 16.
Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed. For complete details on the use and execution of this protocol, please refer to Sun et al. (2022).
激光诱导的非人类灵长类动物高血压被用于模拟人类青光眼，这是导致不可逆转失明的主要原因。在本方案中，我们详细介绍了通过激光光凝小梁网和随后的房内注射来诱导非人类灵长类动物眼内高压的步骤。我们进一步描述了眼压变化和视乳头视网膜神经纤维层厚度的记录和评估。本方案可以帮助研究人员提高手术的成功率和可重复性，并减少所需非人类灵长类动物的数量。有关该方案的使用和执行的完整详细信息，请参阅 Sun 等人。(2022 年)。
