State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, CAS Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing South Road 40-1, Urumqi, 830011, Xinjiang, PR China; University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Shijingshan District, Beijing, 100049, PR China.
State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, CAS Key Laboratory of Chemistry of Plant Resources in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing South Road 40-1, Urumqi, 830011, Xinjiang, PR China.
J Ethnopharmacol. 2023 Feb 10;302(Pt A):115884. doi: 10.1016/j.jep.2022.115884. Epub 2022 Oct 30.
Artiri La Li Honey Pill (ALLHP) is a traditional medicinal formula that is widely used in Xinjiang, China, for the treatment of vitiligo. Since the cause of vitiligo has not been determined, no satisfactory treatment is available. Clinical interventions include pharmacological treatment with psoralen, usually in conjunction with ultraviolet A (UVA) radiation, but toxic side effects limit this application. Studies on the activity and mechanisms of ALLHP are scarce.
To verify the therapeutic effect of ALLHP on vitiligo and determine its effectiveness as a theoretical and experimental basis for the development of innovative drugs with independent intellectual property rights and the effective use of local resources.
The experimental animal model of vitiligo was established by chemical decoloring. Rats were treated with gradient doses of ALLHP. The therapeutic effect was judged by gross observation. The contents of TYR, MAO, AchE and MDA in serum and skin tissue, the number of hair follicles containing melanin in skin tissue, the distribution of epidermal melanin, and the weight index of immune organs were detected, and the therapeutic effect of ALLHP on vitiligo was evaluated. In addition, certain monomer components in ALLHP were used to intervene in the zebrafish juvenile melanin suppression model, and the melanin-activating activities of some monomer components in ALLHP were screened by counting the melanin area ratio.
ALLHP increased the number of melanin-containing hair follicles and the epidermal melanin content in the skin of experimental vitiligo animals, repaired the skin cell morphology to a certain extent, increased the content of TYR in serum and skin, and reduced the content of MDA, AchE and MAO. Carvone, Luteolin, Psoralen and Psoraleae phenol and Bakuchiol could increase the melanin area of experimental melanin inhibition in zebrafish.
According to the results of this study, ALLHP can increase the number of melanin-containing hair follicles and the epidermal melanin content in the skin of vitiligo animals and restore skin cell morphology to a certain extent by reducing oxidative stress in epidermal tissue. A wide range of active ingredients may promote melanogenesis with ALLHP.
阿提里拉·利蜂蜜丸（ALLHP）是一种传统的药用配方，在中国新疆广泛用于治疗白癜风。由于白癜风的病因尚未确定，因此尚无满意的治疗方法。临床干预包括使用补骨脂素进行药理学治疗，通常与紫外线 A（UVA）辐射结合使用，但毒性副作用限制了这种应用。关于 ALLHP 的活性和机制的研究很少。
验证 ALLHP 治疗白癜风的疗效，并确定其作为开发具有自主知识产权的创新药物和有效利用当地资源的理论和实验基础的有效性。
通过化学脱色建立白癜风实验动物模型。用 ALLHP 梯度剂量处理大鼠。通过肉眼观察判断治疗效果。检测血清和皮肤组织中 TYR、MAO、AchE 和 MDA 的含量、皮肤组织中含黑色素的毛囊数量、表皮黑色素分布以及免疫器官的重量指数，并评价 ALLHP 对白癜风的治疗作用。此外，在斑马鱼幼鱼黑色素抑制模型中使用 ALLHP 的某些单体成分进行干预，并通过计数黑色素面积比筛选 ALLHP 中某些单体成分的黑色素激活活性。
ALLHP 增加了实验性白癜风动物皮肤中含黑色素的毛囊数量和表皮黑色素含量，在一定程度上修复了皮肤细胞形态，增加了血清和皮肤中 TYR 的含量，降低了 MDA、AchE 和 MAO 的含量。柠檬烯、木犀草素、补骨脂素、补骨脂酚和补骨脂素醇可增加实验性黑色素抑制斑马鱼的黑色素面积。
根据本研究结果，ALLHP 可通过减少表皮组织中的氧化应激，增加白癜风动物皮肤中含黑色素的毛囊数量和表皮黑色素含量，并在一定程度上恢复皮肤细胞形态。广泛的活性成分可能通过 ALLHP 促进黑色素生成。
