Rheumatology Unit, Department of Medicine-DIMED, University of Padova;
Rheumatology Unit, Department of Medicine-DIMED, University of Padova.
J Vis Exp. 2022 Oct 20(188). doi: 10.3791/64351.
Synovial fluid (SF) analysis is important in diagnosing osteoarthritis (OA). Macroscopic and microscopic features, including total and differential white blood cell (WBC) count, help define the non-inflammatory nature of SF, which is a hallmark of OA. In patients with OA, WBC in SF samples usually does not exceed 2000 cells per microliter, and the percentage of inflammatory cells, such as neutrophils, is very low or absent. Calcium crystals are frequent in SF collected from OA patients. Although their role in the pathogenesis of OA remains unclear, they have been associated with a mild inflammatory process and a more severe disease progression. Recently, calcium crystals have been described in both the early and late stages of OA, indicating that they may play a vital role in diagnosing different clinical subsets of OA and pharmacological treatment. The overall goal of SF analysis in OA is two-fold: to ascertain the non-inflammatory degree of SF and to highlight the presence of calcium crystals.
滑液分析在骨关节炎（OA）的诊断中很重要。宏观和微观特征，包括总白细胞（WBC）计数和分类计数，有助于确定滑液的非炎症性质，这是 OA 的一个标志。在 OA 患者中，滑液样本中的 WBC 通常不超过每微升 2000 个细胞，炎症细胞（如中性粒细胞）的百分比非常低或不存在。OA 患者滑液中经常出现钙晶体。尽管它们在 OA 发病机制中的作用尚不清楚，但它们与轻度炎症过程和更严重的疾病进展有关。最近，OA 的早期和晚期都描述了钙晶体，表明它们可能在诊断 OA 的不同临床亚型和药物治疗中发挥重要作用。OA 中滑液分析的总体目标有两个：确定滑液的非炎症程度，并强调钙晶体的存在。
