Ashley Jon, Potts Indiia G, Olorunniji Femi J
School of Pharmaceutical and Biomolecular Sciences, Liverpool John Moores University, James Parsons Building, 3 Byrom St, Liverpool, L3 3AF, UK.
Chembiochem. 2023 Mar 1;24(5):e202200510. doi: 10.1002/cbic.202200510. Epub 2022 Nov 24.
The use of polymerase enzymes in biotechnology has allowed us to gain unprecedented control over the manipulation of DNA, opening up new and exciting applications in areas such as biosensing, polynucleotide synthesis, and DNA storage, aptamer development and DNA-nanotechnology. One of the most intriguing enzymes which has gained prominence in the last decade is terminal deoxynucleotidyl transferase (TdT), which is one of the only polymerase enzymes capable of catalysing the template independent stepwise addition of nucleotides onto an oligonucleotide chain. This unique enzyme has seen a significant increase in a variety of different applications. In this review, we give a comprehensive discussion of the unique properties and applications of TdT as a biotechnology tool, and the application in the enzymatic synthesis of poly/oligonucleotides. Finally, we look at the increasing role of TdT enzyme in biosensing, DNA storage, synthesis of DNA nanostructures and aptamer development, and give a future outlook for this technology.
聚合酶在生物技术中的应用使我们能够以前所未有的方式控制DNA的操作，为生物传感、多核苷酸合成、DNA存储、适体开发和DNA纳米技术等领域开辟了新的、令人兴奋的应用。在过去十年中备受瞩目的最具吸引力的酶之一是末端脱氧核苷酸转移酶（TdT），它是唯一能够催化在寡核苷酸链上进行不依赖模板的核苷酸逐步添加的聚合酶之一。这种独特的酶在各种不同的应用中得到了显著增加。在本综述中，我们全面讨论了TdT作为生物技术工具的独特性质和应用，以及在聚/寡核苷酸酶促合成中的应用。最后，我们探讨了TdT酶在生物传感、DNA存储、DNA纳米结构合成和适体开发中日益重要的作用，并对该技术的未来进行了展望。
