Souza Luiz Henrique Mesquita, Dias Ianca Rosa, von Zuben de Valega Negrão Cyro, Oliveira Henrique da Costa, Turrini Paula Cristina Gasperazzo, Martins André Guilherme da Costa, Dos Reis João Lucas Maehara Said, Yepez Eric André Velasco, Gobara Bruno Nobuya Katayama, Perecin Caio José, Pesquero João Bosco, Verona Bruno Marinaro, Cerize Natália Neto Pereira
Institute for Technological Research (IPT)-Bionanomanufacturing Center (BIONANO), São Paulo 05508-901, SP, Brazil.
ACS Omega. 2025 May 14;10(20):20452-20464. doi: 10.1021/acsomega.5c00645. eCollection 2025 May 27.
Leveraging the well-established properties of gold nanoparticle (AuNP)-DNA conjugates, this research explored a novel methodology for controlled enzymatic DNA synthesis using gold nanoparticle (AuNP)-DNA conjugates as a solid platform. To this end, Hybrid Nanoparticles (HNPs) were meticulously engineered through the functionalization of AuNPs with rationally designed DNA initiator molecules. These initiator molecules, strategically attached to the AuNP surface, served as a physical support and starting point for DNA extension by the Terminal Deoxynucleotidyl Transferase (TdT) enzyme. The results confirmed the synthesis of homopolymeric DNA extensions on these HNPs (58.27 nm, PDI < 0.2), demonstrating the viability of HNPs as a platform for enzymatic DNA elongation. Although the growing demand for data storage suggests a potential application, this research established the foundational feasibility of enzymatic DNA synthesis on HNPs. While high-density DNA data storage requires extensive development, the demonstrated enzymatic synthesis on AuNP-DNA conjugates warrants significant further exploration for future applications in biotechnology and nanotechnology.
利用金纳米颗粒（AuNP）-DNA 共轭物已确立的特性，本研究探索了一种以金纳米颗粒（AuNP）-DNA 共轭物为固体平台进行可控酶促 DNA 合成的新方法。为此，通过用合理设计的 DNA 引发分子对金纳米颗粒进行功能化，精心构建了杂化纳米颗粒（HNP）。这些引发分子战略性地附着在金纳米颗粒表面，作为末端脱氧核苷酸转移酶（TdT）进行 DNA 延伸的物理支撑和起始点。结果证实了在这些 HNP（58.27 nm，PDI < 0.2）上合成了同聚物 DNA 延伸，证明了 HNP 作为酶促 DNA 延伸平台的可行性。尽管对数据存储的需求不断增长暗示了其潜在应用，但本研究确立了在 HNP 上进行酶促 DNA 合成的基本可行性。虽然高密度 DNA 数据存储需要大量的开发工作，但在 AuNP-DNA 共轭物上已证明的酶促合成值得在生物技术和纳米技术的未来应用中进行进一步深入探索。
