Centro de Investigación y Estudios Avanzados en Odontología "Dr. Keisaburo Miyata," Facultad de Odontología, Universidad Autónoma del Estado de México. Paseo Tollocan esq. Jesús Carranza, Col. Universidad, CP 50130. Toluca, Estado de México, México.
Universidad Nacional Autónoma de México. Blv. UNAM 2011, Col. Predio del Saucillo y el Potrero, Comunidad de los Tepetates, Leon CP 37684, Guanajuato, México.
Medicine (Baltimore). 2022 Nov 4;101(44):e31434. doi: 10.1097/MD.0000000000031434.
Several efforts have been made to improve the glass ionomer cements (GICs) properties with nanotechnology. Fluoride release in once of most beneficial properties of GICs. The purpose of this study was to evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with titanium dioxide nanoparticles (TiO2N).
Evaluate the fluoride release, recharge, and cytotoxicity in GICs reinforced with TiO2N.
Four GICs, FUJI IX EXTRA (G1c), KETAC MOLAR (G2c), IONOFILL MOLAR (G3c), and FUJI IX (G4c) were combined with TiO2N (G1e, G2e, G3e, and G4e) and divided into blocks of 5-mm width and 1-mm thickness 10 each. A total of 80 samples were arranged as follows: GICs alone as negative control (n = 40) and GICs + TiO2N as experimental groups (n = 40). The fluoride release was determined for periods of 1, 2, 6, 10, 31, 90, 180, 240, and 300 days. On days 30 and 179, samples were recharged by submerging in 1 mL of 20,000 ppm sodium fluoride gel. Cytotoxic activity was carried out with gingival fibroblasts, using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide cell viability assay.
The experimental groups obtained the highest and more constant fluoride released when compared to control groups. After the first recharge, experimental groups (G1e, G3e, and G4e) showed statistically significant results (P = .001, 0.010, and 0.001 respectively) enhancing their recharge ability regarding control groups. The second recharge showed better results in G1e concerning the rest of the groups. No cytotoxic activity was observed in all experimental groups, although significant differences were observed in G3e and G4e regarding control group.
The incorporation of TiO2N enhance the fluoride release in glass ionomers with a noncytotoxic effect on human gingival fibroblasts.
已有多项研究尝试利用纳米技术改善玻璃离子水门汀（GIC）的性能。氟化物释放是 GIC 最有益的特性之一。本研究旨在评估纳米二氧化钛（TiO2N）增强型 GIC 的氟化物释放、再补充和细胞毒性。
评估纳米二氧化钛增强型 GIC 的氟化物释放、再补充和细胞毒性。
将 4 种 GIC（FUJI IX EXTRA [G1c]、KETAC MOLAR [G2c]、IONOFILL MOLAR [G3c]和 FUJI IX [G4c]）与 TiO2N（G1e、G2e、G3e 和 G4e）结合，每组各制成 5mm 宽、1mm 厚的 10 个块状物。共安排 80 个样本，具体分组如下：单独的 GIC 作为阴性对照组（n=40），GIC+TiO2N 作为实验组（n=40）。在 1、2、6、10、31、90、180、240 和 300 天期间分别测定氟化物释放量。在第 30 天和第 179 天，将样本浸泡在 1ml 浓度为 20,000ppm 的氟化钠凝胶中进行再补充。采用 3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四唑溴盐细胞活力检测法，对牙龈成纤维细胞进行细胞毒性活性检测。
与对照组相比，实验组（G1e、G3e 和 G4e）获得了更高且更稳定的氟化物释放量。第一次再补充后，实验组（G1e、G3e 和 G4e）的结果具有统计学意义（P=0.001、0.010 和 0.001），相对于对照组，其再补充能力得到增强。第二次再补充时，G1e 组的效果优于其他组。所有实验组均未观察到细胞毒性，但 G3e 和 G4e 组与对照组相比，差异有统计学意义。
纳米二氧化钛的掺入增强了玻璃离子水门汀的氟化物释放，对人牙龈成纤维细胞无细胞毒性作用。
