Platica M, Harding W, Hollander V P
Experientia. 1978 Sep 15;34(9):1154-5. doi: 10.1007/BF01922927.
Dithiothreitol, dithioerythritol and bacterial lipopolysaccharides increase optical absorbance and clot Limulus lysate. Purification of dithiothreitol from possible endotoxin contamination by vacuum sublimation or chromatography does not abolish the reaction with lysate. The dithiols reported active here represent the smallest molecules capable of simulating endotoxin in the Limulus test.
二硫苏糖醇、二硫赤藓糖醇和细菌脂多糖可增加吸光度并使鲎试剂凝结。通过真空升华或色谱法从可能的内毒素污染中纯化二硫苏糖醇并不能消除其与鲎试剂的反应。此处报道具有活性的二硫醇是在鲎试剂检测中能够模拟内毒素的最小分子。
