影响鲎试剂敏感性的因素。
Factors affecting the sensitivity of Limulus lysate.
作者信息
Sullivan J D, Watson S W
出版信息
Appl Microbiol. 1974 Dec;28(6):1023-6. doi: 10.1128/am.28.6.1023-1026.1974.
Abstract
Limulus lysate clots when mixed with picogram quantities of endotoxins. The sensitivity of the lysate was improved 100-fold by the removal of an inhibitor and addition of divalent cations. The methods developed in this investigation eliminated much of the seasonable variability of the lysate, improved the heat stability after lyophilization, and made it possible to use the lysate with saline solutions.
摘要
鲎试剂与皮克级内毒素混合时会发生凝集。通过去除一种抑制剂并添加二价阳离子，鲎试剂的灵敏度提高了100倍。本研究中开发的方法消除了鲎试剂大部分的季节性变异性，提高了冻干后的热稳定性，并使得鲎试剂能够与盐溶液一起使用。
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