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1
Factors affecting the sensitivity of Limulus lysate.影响鲎试剂敏感性的因素。
Appl Microbiol. 1974 Dec;28(6):1023-6. doi: 10.1128/am.28.6.1023-1026.1974.
2
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J Lab Clin Med. 1975 Sep;86(3):430-4.
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Carcinoscorpius rotunda cauda amoebocyte lysate for detection of endotoxins--its preparation, stability, sensitivity and comparison with Limulus amoebocyte lysate.用于检测内毒素的圆尾鲎变形细胞溶解物——其制备、稳定性、敏感性及与鲎试剂的比较
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4
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Appl Environ Microbiol. 1979 Nov;38(5):911-5. doi: 10.1128/aem.38.5.911-915.1979.
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Purification and properties of the clotting enzyme from Limulus lysate.
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7
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引用本文的文献

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Rapid determination of bacteriological water quality by using limulus lysate.利用鲎试剂快速测定水质的细菌学质量。
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3
Sensitive quantitation of endotoxin by enzyme-linked immunosorbent assay with monoclonal antibody against Limulus peptide C.使用抗鲎肽C单克隆抗体的酶联免疫吸附测定法对内毒素进行灵敏定量。
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4
Response of several Limulus amoebocyte lysates to native endotoxin present in gonococcal and nongonococcal urethral exudates from human males.几种鲎变形细胞溶解物对来自男性淋病性和非淋病性尿道渗出物中天然内毒素的反应。
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Sensitive assay, based on hydroxy fatty acids from lipopolysaccharide lipid A, for Gram-negative bacteria in sediments.基于脂多糖脂质A中羟基脂肪酸的沉积物中革兰氏阴性菌灵敏检测法。
Appl Environ Microbiol. 1982 Nov;44(5):1170-7. doi: 10.1128/aem.44.5.1170-1177.1982.
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Characterization of Brucella abortus soluble antigen employed in immunoassay.用于免疫测定的流产布鲁氏菌可溶性抗原的特性分析。
J Clin Microbiol. 1980 Apr;11(4):355-62. doi: 10.1128/jcm.11.4.355-362.1980.
10
Limulus amoebocyte lysate assay for detection and quantitation of endotoxin in a small-volume parenteral product.鲎试剂法检测和定量小容量注射剂中的内毒素。
Appl Environ Microbiol. 1980 Sep;40(3):533-8. doi: 10.1128/aem.40.3.533-538.1980.

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DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
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DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.圆盘电泳。一、背景与理论。
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THE ROLE OF ENDOTOXIN IN THE EXTRACELLULAR COAGULATION OF LIMULUS BLOOD.内毒素在鲎血体外凝血中的作用。
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Detection of endotoxin in human blood and demonstration of an inhibitor.人体血液中内毒素的检测及一种抑制剂的证明
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Some characteristics of the clottable protein of limulus polyphemus blood cells.
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The Limulus test for endotoxin (Pyrogen) in radiopharmaceuticals and biologicals.用于检测放射性药物和生物制品中内毒素（热原）的鲎试剂法。
Bull Parenter Drug Assoc. 1972 Jul-Aug;26(4):153-62.
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Gram-negative sepsis: detection of endotoxemia with the limulus test. With studies of associated changes in blood coagulation, serum lipids, and complement.革兰氏阴性菌败血症：用鲎试剂检测内毒素血症。并研究凝血、血脂和补体的相关变化。
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A technique for quantitative measurement of endotoxin in human plasma.一种定量测量人血浆中内毒素的技术。
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Endotoxin and endotoxemia.内毒素与内毒素血症
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An invertebrate coagulation system activated by endotoxin: evidence for enzymatic mediation.一种由内毒素激活的无脊椎动物凝血系统：酶介导的证据。
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影响鲎试剂敏感性的因素。

Factors affecting the sensitivity of Limulus lysate.

作者信息

Sullivan J D, Watson S W

出版信息

Appl Microbiol. 1974 Dec;28(6):1023-6. doi: 10.1128/am.28.6.1023-1026.1974.

DOI:10.1128/am.28.6.1023-1026.1974
PMID:4615633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC186875/
Abstract

Limulus lysate clots when mixed with picogram quantities of endotoxins. The sensitivity of the lysate was improved 100-fold by the removal of an inhibitor and addition of divalent cations. The methods developed in this investigation eliminated much of the seasonable variability of the lysate, improved the heat stability after lyophilization, and made it possible to use the lysate with saline solutions.

摘要

鲎试剂与皮克级内毒素混合时会发生凝集。通过去除一种抑制剂并添加二价阳离子，鲎试剂的灵敏度提高了100倍。本研究中开发的方法消除了鲎试剂大部分的季节性变异性，提高了冻干后的热稳定性，并使得鲎试剂能够与盐溶液一起使用。