Malik Muhammad Haseeb, Moaeen-Ud-Din Muhammad, Bilal Ghulam, Ghaffar Abdul, Muner Raja Danish, Raja Ghazala Kaukab, Khan Waqas Ahmad
University Institute of Biochemistry & Biotechnology, PMAS Arid Agriculture University, Rawalpindi, 46300, Pakistan.
These authors contributed equally to this work.
Arch Anim Breed. 2018 Oct 12;61(4):387-394. doi: 10.5194/aab-61-387-2018. eCollection 2018.
The identification issue of livestock can be resolved by using molecular identification tools that are acceptable to preserve and maintain pure breeds worldwide. The application of a molecular identification methodology is more important for developing nations, e.g., Pakistan, where uncontrolled crossbreeding has become a common practice and the import of exotic animals and germplasm is ever increasing. This presents a risk to local breeds as also stated by the FAO. Therefore, the current study was designed to develop standard molecular markers for Cholistani cattle to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for Cholistani and each crossbred cattle, respectively. Candidate molecular markers present in Cholistani but absent in crossbred cattle and vice versa were detected using the amplified fragment length polymorphism (AFLP) method. Eleven markers were developed and were converted to single nucleotide polymorphism (SNP) markers for genotyping. The allele frequencies in both breeds were determined for discrimination ability using polymerase-chain-reaction-restriction-fragment-polymorphism (PCR-AFLP). The probability of identifying the Cholistani breed was 0.905 and the probability of misjudgment was 0.073 using a panel of markers. The identified markers can ascertain the breed purity and are likely to extend the facility for breed purity testing before entering into a genetic improvement program in the country.
通过使用全球范围内可接受的用于保存和维持纯种的分子识别工具，可以解决牲畜的识别问题。分子识别方法的应用对发展中国家更为重要，例如巴基斯坦，在那里不受控制的杂交已成为常见做法， exotic动物和种质的进口也在不断增加。正如粮农组织所述，这对当地品种构成了风险。因此，本研究旨在为乔利斯坦牛开发标准分子标记，以确定其用于育种目的的纯度。在本研究中，分别对50头和48头无关的雄性乔利斯坦牛和每头杂交牛进行了采样。使用扩增片段长度多态性（AFLP）方法检测乔利斯坦牛中存在但杂交牛中不存在的候选分子标记，反之亦然。开发了11个标记，并将其转换为单核苷酸多态性（SNP）标记用于基因分型。使用聚合酶链反应-限制性片段多态性（PCR-AFLP）确定两个品种的等位基因频率以评估鉴别能力。使用一组标记识别乔利斯坦品种的概率为0.905，误判概率为0.073。所识别的标记可以确定品种纯度，并可能在该国进入遗传改良计划之前扩展品种纯度测试的设施。
