State Forestry and Grassland Administration Key Laboratory of Silviculture in Downstream Areas of the Yellow River, Forestry College of Shandong Agricultural University, Taian, Shandong, China.
Shandong Forest and Grass Germplasm Resources Center, Jinan, Shandong, China.
PeerJ. 2022 Nov 2;10:e14172. doi: 10.7717/peerj.14172. eCollection 2022.
, as an important plant for ecological protection, is a pioneer tree species for afforestation in arid and barren mountainous areas. Lignin has the functions of water and soil conservation, strengthening plant mechanical strength and resisting adverse environmental effects and plays an important role in the ecological protection benefits of .
In this study, annual dynamic observations of the lignin content in roots, stems and leaves of one-year-old seedlings of a half-sib family were carried out, and combined transcriptome and metabolome analyses were carried out during three key stages of stem development.
The lignin contents in roots, stems and leaves of showed extremely significant spatiotemporal differences. In the stems, lignin was mainly distributed in the cell walls of the pith, xylem, phloem, pericyte, and epidermis, with differences in different periods. A total of 226 metabolites were detected in the stem of , which were divided into seven categories, including 10 synthetic precursor compounds containing lignin. Among them, the content of coniferyl alcohol was the highest, accounting for 12.27% of the total content, and caffeyl alcohol was the lowest, accounting for 7.05% only. By annotating the KEGG functions, a large number of differentially expressed genes and differential metabolites were obtained for the comparison combinations, and seven key enzymes and 24 related genes involved in the process of lignin synthesis in were selected.
Based on the results of the metabolic mechanism of lignin in by biochemical, anatomical and molecular biological analyzes, the key regulatory pathways of lignin in were identified, which will be of great significance for regulating the lignin content of and improving the adaptability and resistance of this plant.
作为生态保护的重要植物，是干旱荒山造林的先锋树种。木质素具有保持水土、增强植物机械强度和抵抗不良环境影响的功能，在的生态保护效益中起着重要作用。
本研究对一年生半同胞家系幼苗的根、茎、叶中的木质素含量进行了年度动态观测，并在茎发育的三个关键阶段进行了转录组和代谢组分析。
表现出根、茎、叶木质素含量具有极显著的时空差异。在茎中，木质素主要分布在髓、木质部、韧皮部、周皮和表皮的细胞壁中，不同时期存在差异。共检测到 226 种代谢物，分为 7 类，其中包含 10 种木质素合成前体化合物。其中松柏醇含量最高，占总含量的 12.27%，咖啡醇含量最低，仅占 7.05%。通过注释 KEGG 功能，比较组合得到了大量差异表达基因和差异代谢物，选择了 7 种关键酶和 24 种与木质素合成过程相关的基因。
通过对木质素代谢机制的生化、解剖和分子生物学分析，确定了木质素在中的关键调控途径，这对于调节木质素含量和提高该植物的适应性和抗性具有重要意义。
