Gu Junjun, Shang Endong, Ma Miao
Key Laboratory of Oasis Town and Mountain-basin System Ecology, Key Laboratory of Xinjiang Phytomedicine Resource Utilization, Ministry of Education, College of life Sciences, Shihezi University, Shihezi 832003, China.
Plants (Basel). 2025 Aug 20;14(16):2589. doi: 10.3390/plants14162589.
The rust disease caused by seriously affects the growth of However, the defense mechanisms against rust infection remain unclear. This study explored the regulatory mechanisms of in response to rust disease through combined physiological, biochemical, and transcriptomic analyses. The results showed that with the increase in disease severity, the chlorophyll content of leaves decreased significantly, while the antioxidant and phenylalanine ammonia lyase activities progressively increased. Mild infection triggered an 11.9-fold surge in salicylic acid levels and a sharp decline in abscisic acid compared to controls, as well as increased synthesis of total phenolics, total flavonoids, chlorogenic acid, cryptochlorogenic acid, isoquercetin, hyperoside, rutin, and astragalin. Transcriptome analysis showed that the "plant-pathogen interaction, plant hormone signal transduction and phenylpropanoid biosynthesis" pathways were significantly up-regulated in the mild infection stage, while "glycerophospholipid metabolism, fatty acid degradation and ABC transporters" were activated in the severe infection stage. In summary, regulates energy metabolism and phenylpropanoid metabolism through salicylic acid signaling and promotes the accumulation of secondary metabolites and the lignification process of leaves, thereby enhancing rust resistance. Key enzyme genes (COMT, POD, CAD, F5H) and metabolites (chlorogenic acid, isoquercitrin, rutin) can be used as important targets for disease resistance breeding. Our research provides important reference for the prevention and control of in .
由[病原体名称未给出]引起的锈病严重影响[植物名称未给出]的生长。然而，针对锈病感染的防御机制仍不清楚。本研究通过生理、生化和转录组学联合分析，探索了[植物名称未给出]对锈病的调控机制。结果表明，随着病害严重程度的增加，叶片叶绿素含量显著降低，而抗氧化剂和苯丙氨酸解氨酶活性逐渐增加。与对照相比，轻度感染引发水杨酸水平激增11.9倍，脱落酸急剧下降，同时总酚、总黄酮、绿原酸、隐绿原酸、异槲皮苷、金丝桃苷、芦丁和黄芪苷的合成增加。转录组分析表明，“植物-病原体相互作用、植物激素信号转导和苯丙烷生物合成”途径在轻度感染阶段显著上调，而“甘油磷脂代谢、脂肪酸降解和ABC转运蛋白”在重度感染阶段被激活。综上所述，[植物名称未给出]通过水杨酸信号调节能量代谢和苯丙烷代谢，促进次生代谢产物的积累和叶片的木质化过程，从而增强对锈病的抗性。关键酶基因（COMT、POD、CAD、F5H）和代谢产物（绿原酸、异槲皮苷、芦丁）可作为抗病育种的重要靶点。我们的研究为[植物名称未给出]锈病的防治提供了重要参考。
