Genomics Research Center, Academia Sinica, Taipei, Taiwan.
Methods Mol Biol. 2023;2552:437-445. doi: 10.1007/978-1-0716-2609-2_24.
To ensure the functionalities of the antibodies in phage-displayed synthetic antibody libraries, we use computational method to evaluate the designs of the antibody libraries. The computational methodologies developed in our lab for designing antibody library provide rich information on the function of the designed antibody sequences-adequate antibody designs for a specific antigen type should have predicted paratopes for the antigen type. This computational assessment of the designed antibody sequences helps eliminate non-functional designs before proceeding to construct the library designs in the wet lab. As such, only reasonable antibody designs are constructed for antibody discoveries.
为确保噬菌体展示合成抗体库中的抗体的功能，我们使用计算方法来评估抗体库的设计。我们实验室开发的用于设计抗体库的计算方法为设计的抗体序列的功能提供了丰富的信息——针对特定抗原类型的适当抗体设计应具有针对该抗原类型的预测表位。对设计的抗体序列进行这种计算评估有助于在进入湿实验室构建文库设计之前消除非功能设计。因此，只有合理的抗体设计才能用于抗体发现。
