Suppr超能文献

减少的线粒体为细胞质蛋氨酸循环提供了必不可少的功能。

Reduced mitochondria provide an essential function for the cytosolic methionine cycle.

机构信息

Charles University, Faculty of Science, Department of Parasitology, BIOCEV, Vestec 252 50, Czech Republic.

Charles University, Faculty of Science, BIOCEV, Vestec 252 50, Czech Republic.

出版信息

Curr Biol. 2022 Dec 5;32(23):5057-5068.e5. doi: 10.1016/j.cub.2022.10.028. Epub 2022 Nov 7.

Abstract

The loss of mitochondria in oxymonad protists has been associated with the redirection of the essential Fe-S cluster assembly to the cytosol. Yet as our knowledge of diverse free-living protists broadens, the list of functions of their mitochondrial-related organelles (MROs) expands. We revealed another such function in the closest oxymonad relative, Paratrimastix pyriformis, after we solved the proteome of its MRO with high accuracy, using localization of organelle proteins by isotope tagging (LOPIT). The newly assigned enzymes connect to the glycine cleavage system (GCS) and produce folate derivatives with one-carbon units and formate. These are likely to be used by the cytosolic methionine cycle involved in S-adenosyl methionine recycling. The data provide consistency with the presence of the GCS in MROs of free-living species and its absence in most endobionts, which typically lose the methionine cycle and, in the case of oxymonads, the mitochondria.

摘要

线粒体在氧化亚铁叶菌原生生物中的缺失与必需的 Fe-S 簇组装重定向到细胞质有关。然而,随着我们对不同自由生活原生生物的认识不断扩大,它们的线粒体相关细胞器(MRO)的功能列表也在不断扩大。在最接近的氧化亚铁叶菌原生生物 Paratrimastix pyriformis 中,我们使用同位素标记定位细胞器蛋白(LOPIT)高精度地解决了其 MRO 的蛋白质组后,揭示了另一个这样的功能。新分配的酶与甘氨酸裂解系统(GCS)连接,并产生带有一碳单位和甲酸盐的叶酸衍生物。这些可能被涉及 S-腺苷甲硫氨酸循环回收的细胞质蛋氨酸循环利用。这些数据与 GCS 在自由生活物种的 MRO 中的存在以及在大多数内生物中的缺失一致,内生物通常失去蛋氨酸循环,而在氧化亚铁叶菌中,还失去了线粒体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6cb2/9746703/6402ea2588fe/fx1.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验