来自野葛的葛根醇及葛根苷衍生物及其抗炎活性。
Puerol and pueroside derivatives from Pueraria lobata and their anti-inflammatory activity.
作者信息
Sun Ying-Jie, Cao Shi-Jie, Liang Feng-Ni, Li Jin-Yan, Zhang Xiang-Yu, Li Wei, Ding Li-Qin, Qiu Feng
机构信息
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, China; State Key Laboratory of Component-based Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China; Tianjin Zhongxin Pharmaceutical Group Co., Ltd. Le Ren Tang Pharmaceutical Factory, Tianjin, China.
State Key Laboratory of Component-based Chinese Medicine, Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
出版信息
Phytochemistry. 2023 Jan;205:113507. doi: 10.1016/j.phytochem.2022.113507. Epub 2022 Nov 5.
Five undescribed puerols and puerosides and three known analogues were obtained from the roots of Pueraria lobata. Their structures were determined by comprehensive analysis of spectroscopic data and chemical methods. Since puerol D and puerol C were racemic compounds, resolved into their enantiomers, and their absolute configurations were determined by experimental and calculated ECD spectra. Six of the isolates were evaluated for their inhibitory activities on NO generation and the expression of inflammatory factors in the LPS-stimulated RAW 264.7 macrophage cells. The results showed that (S)-puerol C, (R)-puerol C, isokuzubutenolide A and kuzubutenolide A significantly decreased the NO production (IC values in the range of 16.87-39.95 μM). Meanwhile, (S)-puerol C, isokuzubutenolide A and kuzubutenolide A also reduced the mRNA expression of inflammatory factors (TNF-α, IL-1β and IL-6).
从野葛的根中获得了5种未描述的葛根醇和葛根苷以及3种已知类似物。通过光谱数据和化学方法的综合分析确定了它们的结构。由于葛根醇D和葛根醇C是外消旋化合物，将其拆分为对映体，并通过实验和计算的电子圆二色光谱确定其绝对构型。评估了其中6种分离物对脂多糖刺激的RAW 264.7巨噬细胞中一氧化氮生成和炎症因子表达的抑制活性。结果表明，(S)-葛根醇C、(R)-葛根醇C、异库祖布内酯A和库祖布内酯A显著降低了一氧化氮的产生（IC值在16.87-39.95μM范围内）。同时，(S)-葛根醇C、异库祖布内酯A和库祖布内酯A也降低了炎症因子（TNF-α、IL-1β和IL-6）的mRNA表达。
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