鉴定位于产朊假丝酵母tRNAPro反密码子第一位的修饰核苷为5-氨甲酰甲基尿苷。
Identification of a modified nucleoside located in the first position of the anticodon of Torulopsis utilis tRNAPro as 5-carbamoylmethyluridine.
作者信息
Ogawa K, Kondo T, Kawakami M, Takemura S
出版信息
J Biochem. 1986 Feb;99(2):527-30. doi: 10.1093/oxfordjournals.jbchem.a135508.
Abstract
An unknown nucleoside in the first position of the anticodon of Torulopsis utilis tRNAPro has been isolated. The UV, 1H NMR and secondary ion mass spectra indicated that this nucleoside is a uridine derivative, 5-carbamoylmethyluridine. The structure was completely established by comparison of the instrumental analysis results and chromatographic behavior of the isolated nucleoside with those of a synthetic sample.
摘要
已从产朊假丝酵母tRNAPro反密码子第一位分离出一种未知核苷。紫外光谱、1H核磁共振光谱和二次离子质谱表明，该核苷是一种尿苷衍生物，即5-氨甲酰甲基尿苷。通过将分离核苷的仪器分析结果和色谱行为与合成样品的进行比较，完全确定了其结构。
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