Biniaz Yaser, Khalesi Mohammadreza, Niazi Ali, Afsharifar Alireza
Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran.
Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran.
Amino Acids. 2023 Jan;55(1):19-31. doi: 10.1007/s00726-022-03200-1. Epub 2022 Nov 8.
Plant viral pathogens cause damaging diseases in many agriculture systems, and emerging viral infections are a serious threat for providing adequate food to a continuously growing population. Recent studies of biogenic substances have provided new opportunities for producing novel antiviral agents. The present work has been conducted to evaluate the antiviral activity of quinoa (Chenopodium quinoa Willd.) seeds crude extract. The antiviral activity was retained in different buffer solutions of various pH ranges (5.2-8.5) and remained after the diafiltration process. The putative virus inhibitor was sensitive to treatment with sodium dodecyl sulfate and trichloroacetic acid. An antiviral protein with ~ 25 kDa molecular weight was isolated from the seed quinoa extract using ammonium sulfate precipitation, anion and cation exchange chromatography. The purified protein (Quinoin-I) significantly inhibited TMV on tobacco leaves with an IC value at a 6.81 μg/ml concentration. Enzyme activity assay revealed the RNase activity of Quinoin-I, and this feature was retained in the presence of β-mercaptoethanol and ethylene diamine tetraacetic acid. This antiviral protein has been shown as a promising leading molecule for further development as a novel antiviral agent.
植物病毒病原体在许多农业系统中引发破坏性疾病，新出现的病毒感染对为持续增长的人口提供充足食物构成严重威胁。最近对生物源物质的研究为生产新型抗病毒药物提供了新机会。本研究旨在评估藜麦（Chenopodium quinoa Willd.）种子粗提物的抗病毒活性。抗病毒活性在不同pH范围（5.2 - 8.5）的缓冲溶液中得以保留，并且在渗滤过程后仍然存在。推测的病毒抑制剂对十二烷基硫酸钠和三氯乙酸处理敏感。使用硫酸铵沉淀、阴离子和阳离子交换色谱从藜麦种子提取物中分离出一种分子量约为25 kDa的抗病毒蛋白。纯化后的蛋白（藜麦素 - I）在浓度为6.81 μg/ml时对烟草叶片上的烟草花叶病毒（TMV）具有显著抑制作用，IC值表明了这一点。酶活性测定显示藜麦素 - I具有核糖核酸酶活性，并且在存在β - 巯基乙醇和乙二胺四乙酸的情况下该特性得以保留。这种抗病毒蛋白已被证明是一种有前途的先导分子，可进一步开发成为新型抗病毒药物。
