Obara Katsuya, Obara Henmi Chizuka, Naito Mitsuru, Mitsui Ikki, Une Yumi, Asano Atsushi, Tajima Atsushi
Takanedai Animal Clinic, Narashinodai 2-16-3, Funabashi, Chiba 274-0063, Japan.
Graduate School of Science and Technology, University of Tsukuba, Ten-noh Dai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
J Poult Sci. 2022 Oct 25;59(4):371-377. doi: 10.2141/jpsa.0220031.
The effects of oxygen gas injection starting on day 17 of incubation (D17) in a chick shell-less culture system (cSLC) on the subsequent embryo development were examined on day 19 of incubation (D19). On D19 of cSLC, the plasma phosphorus and total cholesterol concentrations of the embryos were significantly higher (<0.05), while the plasma calcium concentrations were significantly lower (<0.05) than those in the intact control (IC) group. However, no significant differences in embryo viability and other major blood component levels were observed among the experimental groups (>0.05). The percutaneous oxygen saturation was lower in D17-cSLC embryos before oxygen gas supplementation than in the IC (<0.05) embryos. Severe renal tubular degeneration of the metanephros was observed in D19-cSLC embryos despite oxygen gas injection starting from D17. These results indicate that D19-cSLC embryos are hypoxia even after injecting oxygen gas starting on D17.
在鸡无壳培养系统（cSLC）中，从孵化第17天（D17）开始注入氧气对后续胚胎发育的影响在孵化第19天（D19）进行了检查。在cSLC的D19时，胚胎的血浆磷和总胆固醇浓度显著更高（<0.05），而血浆钙浓度显著更低（<0.05），与完整对照组（IC）相比。然而，实验组之间在胚胎活力和其他主要血液成分水平上未观察到显著差异（>0.05）。在补充氧气之前，D17-cSLC胚胎的经皮氧饱和度低于IC（<0.05）胚胎。尽管从D17开始注入氧气，但在D19-cSLC胚胎中观察到后肾严重的肾小管变性。这些结果表明，即使从D17开始注入氧气，D19-cSLC胚胎仍处于缺氧状态。
