Obara Katsuya, Obara Henmi Chizuka, Naito Mitsuru, Mitsui Ikki, Une Yumi, Asano Atsushi, Tajima Atsushi
Takanedai Animal Clinic, Narashinodai 2-16-3, Funabashi, Chiba 274-0063, Japan.
Graduate School of Science and Technology, University of Tsukuba, Ten-noh Dai 1-1-1, Tsukuba, Ibaraki 305-8572, Japan.
J Poult Sci. 2022 Apr 25;59(2):168-174. doi: 10.2141/jpsa.0210024.
This study examined the effects of calcium lactate on the development of chicken embryos in a shell-less culture system (cSLCS) up to the seventeenth day of incubation. In the presence of calcium lactate, a significant reduction in embryo viability was observed during the first week of incubation in cSLCS. On day 17 of embryo development, no significant difference was observed in the blood plasma calcium concentration or tibia bone density between cSLCS and intact control embryos, whereas the tibia length was significantly shorter in cSLCS embryos than in the intact control. These results suggest that calcium lactate supplementation in cSLCS supports bone formation in developing chicken embryos, but has adverse effects on the viability of embryos, particularly during the first week of embryo development.
本研究考察了乳酸钙对无壳培养系统（cSLCS）中鸡胚胎发育至孵化第17天的影响。在乳酸钙存在的情况下，观察到cSLCS孵化第一周胚胎活力显著降低。在胚胎发育第17天，cSLCS胚胎与完整对照胚胎之间的血浆钙浓度或胫骨骨密度无显著差异，而cSLCS胚胎的胫骨长度显著短于完整对照。这些结果表明，在cSLCS中补充乳酸钙有助于发育中的鸡胚胎骨骼形成，但对胚胎活力有不利影响，尤其是在胚胎发育的第一周。
