Winkler Steffen, Meyer Katharina V, Heuer Christopher, Kortmann Carlotta, Dehne Michaela, Bahnemann Janina
Institute of Technical Chemistry Leibniz University Hannover Hannover Germany.
Cell Culture Technology Faculty of Technology Bielefeld University Bielefeld Germany.
Eng Life Sci. 2022 Mar 31;22(11):699-708. doi: 10.1002/elsc.202100104. eCollection 2022 Nov.
Additive manufacturing (3D printing) enables the fabrication of highly customized and complex devices and is therefore increasingly used in the field of life sciences and biotechnology. However, the application of 3D-printed parts in these fields requires not only their biocompatibility but also their sterility. The most common method for sterilizing 3D-printed parts is heat steam sterilization-but most commercially available 3D printing materials cannot withstand high temperatures. In this study, a novel heat-resistant polyacrylate material for high-resolution 3D Multijet printing was evaluated for the first time for its resistance to heat steam sterilization and in vitro biocompatibility with mouse fibroblasts (L929), human embryonic kidney cells (HEK 293E), and yeast ( ()). Analysis of the growth and viability of L929 cells and the growth of confirmed that the extraction media obtained from 3D-printed parts had no negative effect on the aforementioned cell types, while, in contrast, viability and growth of HEK 293E cells were affected. No different effects of the material on the cells were found when comparing heat steam sterilization and disinfection with ethanol (70%, v/v). In principle, the investigated material shows great potential for high-resolution 3D printing of novel cell culture systems that are highly complex in design, customized and easily sterilizable-however, the biocompatibility of the material for other cell types needs to be re-evaluated.
增材制造（3D打印）能够制造高度定制化和复杂的设备，因此在生命科学和生物技术领域的应用越来越广泛。然而，3D打印部件在这些领域的应用不仅需要其生物相容性，还需要其无菌性。对3D打印部件进行灭菌的最常用方法是热蒸汽灭菌——但大多数市售的3D打印材料无法承受高温。在本研究中，首次对一种用于高分辨率3D多喷射打印的新型耐热聚丙烯酸酯材料进行了热蒸汽灭菌抗性以及与小鼠成纤维细胞（L929）、人胚肾细胞（HEK 293E）和酵母（）的体外生物相容性评估。对L929细胞的生长和活力以及的生长分析证实，从3D打印部件获得的提取培养基对上述细胞类型没有负面影响，而相比之下，HEK 293E细胞的活力和生长受到了影响。在比较热蒸汽灭菌和用乙醇（70%，v/v）消毒时，未发现该材料对细胞有不同影响。原则上，所研究的材料在设计高度复杂、定制化且易于灭菌的新型细胞培养系统的高分辨率3D打印方面显示出巨大潜力——然而，该材料对其他细胞类型的生物相容性需要重新评估。
