Transcriptome-wide profiling of -methyladenosine a selective chemical labeling method.

Studies of chemical modifications on RNA have ushered in the field of epitranscriptomics. -Methyladenosine (mA) is the most typical RNA modification and is indispensable for basic biological processes. This study presents a chemical pulldown method (mA-ORL-Seq) for transcriptome-wide profiling of mA. Moreover, chemical labeling results in a specific reverse transcription (RT) truncation signature. This study has identified four thousand high-confidence mA sites at single-base resolution in the human transcriptome. Unlike previously reported methods based on mA-antibody or mA-sensitive enzymes, the antibody/enzyme-free chemical method provides a new perspective for single-base mA detection at the transcriptome level.