NT-seq：一种基于化学的全基因组甲基化组测序方法。

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

Genetics, Genomics, and Bioinformatics Graduate Program, University of California Riverside, Riverside, CA, USA.

Genome Biol. 2022 May 30;23(1):122. doi: 10.1186/s13059-022-02689-9.

DNA methylation plays vital roles in both prokaryotes and eukaryotes. There are three forms of DNA methylation in prokaryotes: N-methyladenine (6mA), N-methylcytosine (4mC), and 5-methylcytosine (5mC). Although many sequencing methods have been developed to sequence specific types of methylation, few technologies can be used for efficiently mapping multiple types of methylation. Here, we present NT-seq for mapping all three types of methylation simultaneously. NT-seq reliably detects all known methylation motifs in two bacterial genomes and can be used for identifying de novo methylation motifs. NT-seq provides a simple and efficient solution for detecting multiple types of DNA methylation.

DNA 甲基化在原核生物和真核生物中都起着至关重要的作用。原核生物中有三种形式的 DNA 甲基化：N6-甲基腺嘌呤（6mA）、N4-甲基胞嘧啶（4mC）和 5-甲基胞嘧啶（5mC）。尽管已经开发了许多测序方法来测序特定类型的甲基化，但很少有技术可用于有效地绘制多种类型的甲基化图谱。在这里，我们提出了 NT-seq 用于同时绘制所有三种类型的甲基化。NT-seq 可靠地检测到两个细菌基因组中所有已知的甲基化基序，并且可用于鉴定新的甲基化基序。NT-seq 为检测多种类型的 DNA 甲基化提供了一种简单有效的解决方案。