Okada Yoshikiyo, Sugihara Nao, Nishii Shin, Itoh Suguru, Mizoguchi Akinori, Tanemoto Rina, Horiuchi Kazuki, Tomioka Akira, Nishimura Hiroyuki, Higashiyama Masaaki, Narimatsu Kazuyuki, Kurihara Chie, Tomita Kengo, Miura Soichiro, Tsuzuki Yoshikazu, Hokari Ryota
Department of Internal Medicine, National Defense Medical College, Tokorozawa, Saitama, Japan.
Graduate School, International University of Health and Welfare, Tokyo, Japan.
J Gastroenterol Hepatol. 2023 Feb;38(2):311-320. doi: 10.1111/jgh.16058. Epub 2022 Nov 21.
The study of the impact of environmental factors during pregnancy on fetal development has so far been focused primarily on those negatively affecting human health; however, little is known about the effects of probiotic treatment during pregnancy on inflammatory bowel diseases (IBD). In this study, we investigated whether oral administration of heat-killed probiotics isolated from fermented foods decreased the vulnerability of offspring to IBD.
Probiotics were administered to the pregnant mice until the birth of pups, after which the parent mice were maintained with autoclaved water. Partial pups were evaluated for dextran sodium sulfate-induced colitis. The influence of CD11c CD103 dendritic cells (DCs) and regulatory T cells (Tregs) in mesenteric lymph nodes of parent mice and their pups was analyzed.
Oral administration of heat-killed probiotics to pregnant dams significantly decreased inflammation induced by dextran sodium sulfate in pups. Probiotic treatment increased the number of CD103 DCs, and the expression of β8-integrin in CD103 DCs and Tregs in mesenteric lymph nodes, not only in dams themselves but also in their offspring.
Oral administration of probiotics during gestation induced transgenerational immunomodulatory effects on the gut-associated immune system and resilience to experimental colitis in the offspring. Our results suggest that consumption of fermented foods during pregnancy can be effective in preventing inflammatory diseases such as IBD beyond generation.
迄今为止，关于孕期环境因素对胎儿发育影响的研究主要集中在那些对人类健康有负面影响的因素上；然而，孕期益生菌治疗对炎症性肠病（IBD）的影响却知之甚少。在本研究中，我们调查了口服从发酵食品中分离出的热灭活益生菌是否能降低后代患IBD的易感性。
在怀孕小鼠分娩前一直给其服用益生菌，之后亲代小鼠用高压灭菌水饲养。部分幼崽接受葡聚糖硫酸钠诱导的结肠炎评估。分析亲代小鼠及其幼崽肠系膜淋巴结中CD11c CD103树突状细胞（DCs）和调节性T细胞（Tregs）的影响。
给怀孕母鼠口服热灭活益生菌可显著降低幼崽中葡聚糖硫酸钠诱导的炎症。益生菌治疗增加了CD103 DCs的数量，以及肠系膜淋巴结中CD103 DCs和Tregs中β8整合素的表达，不仅在母鼠自身，在其后代中也是如此。
孕期口服益生菌可对肠道相关免疫系统产生跨代免疫调节作用，并增强后代对实验性结肠炎的抵抗力。我们的结果表明，孕期食用发酵食品可有效预防IBD等炎症性疾病的隔代发生。
