Lobanok T E, Pesniakevich A G, Fomichev Iu K
Genetika. 1978;14(12):2119-27.
The conjugal transfer of seven R factors with different genes of drug resistance from Escherichia coli bacteria to twenty Erwinia strains was studied. Seven Erwinia strains were able to accept R plasmids, however the frequency of R factors transfer from E. coli to Erwinia was about 10(-6)-10(-4), and as a rule was higher when the cross was made at optimum temperature for the donor. The most of R- exconjugants showed higher degree of resistance to streptomycin and lower degrees of resistance to tetracycline and chloramphenicol than donor E. coli strains. R plasmids which were transferred to Erwinia from enteric bacteria were characterized by low genetical stability and in some cases were eliminated at first generations of recipient bacteria. Some R- strains of Erwinia exconjugants were able to transfer the accepted plasmids by conjugation into E. coli cells, but were not able to do it in homologous systems.
研究了7种携带不同耐药基因的R因子从大肠杆菌向20株欧文氏菌的接合转移。7株欧文氏菌能够接受R质粒,然而R因子从大肠杆菌转移到欧文氏菌的频率约为10^(-6)-10^(-4),通常在供体的最适温度下进行杂交时频率更高。大多数R-接合子对链霉素的耐药程度高于供体大肠杆菌菌株,对四环素和氯霉素的耐药程度较低。从肠道细菌转移到欧文氏菌的R质粒具有低遗传稳定性,在某些情况下在受体细菌的第一代就被消除。一些R-欧文氏菌接合子菌株能够通过接合将接受的质粒转移到大肠杆菌细胞中,但在同源系统中则不能。