Murakami Satoshi, Adachi Hiroaki, Matsumura Hiroyoshi, Takano Kazufumi, Inoue Tsuyoshi, Mori Yusuke
Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan.
SOSHO Inc., Suita, Osaka 565-0871, Japan.
J Biochem. 2023 Feb 3;173(2):65-72. doi: 10.1093/jb/mvac087.
Polyacrylamide gel electrophoresis (PAGE) with sodium dodecyl sulphate (SDS) and Coomassie brilliant blue (CBB) staining is widely used in protein research and requires time for electrophoresis, staining and destaining. Because the protein bands electrophoresed in the gel are invisible in most cases, the results cannot be observed until the whole process is complete. In this study, shadowgraph was applied to detect biomolecules such as proteins during electrophoresis. A simple optical system and camera-enabled real-time monitoring of migration and separation of individual protein bands in polyacrylamide gels without staining. The visibility was high enough that it was possible to visualize substances other than proteins, such as DNA. This method provides protein profiles instantly in the early stage of electrophoresis. The elimination of the staining and destaining steps will help save researchers' time. The method is also environmentally friendly and will help reduce the generation of waste solutions containing synthetic dyes.
十二烷基硫酸钠（SDS）聚丙烯酰胺凝胶电泳（PAGE）和考马斯亮蓝（CBB）染色在蛋白质研究中被广泛应用，且电泳、染色和脱色都需要时间。由于大多数情况下在凝胶中电泳的蛋白质条带是不可见的，所以直到整个过程完成才能观察到结果。在本研究中，利用投影成像在电泳过程中检测蛋白质等生物分子。一个简单的光学系统和相机能够实时监测聚丙烯酰胺凝胶中单个蛋白质条带的迁移和分离情况，无需染色。可见度足够高，以至于除蛋白质外的其他物质如DNA也能被可视化。该方法在电泳早期就能即时提供蛋白质图谱。省去染色和脱色步骤将有助于节省研究人员的时间。该方法还环保，有助于减少含合成染料废液的产生。
