Huang Ke, Meng Yucheng, Lu Jiyuan, Xu Lingdan, Wang Shiqi, Wang Huihui, Xu Zhaoqing
Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Gansu, China.
Key Laboratory of Dental Maxillofacial Reconstruction and Biological Intelligence Manufacturing, School of Stomatology, Lanzhou University, Gansu, China.
Front Genet. 2022 Oct 24;13:1050402. doi: 10.3389/fgene.2022.1050402. eCollection 2022.
Tight junction-associated marvel proteins (TAMP) is a transmembrane protein whose members are associated with tight junctions between cells and epithelial remodeling. MARVEL domain containing 3 () is one of the members of the TAMP. , as a novel tight junction protein involved in bicellular tight junction assembly, has attracted growing attention in the field of oncology. This study aimed to investigate the prognostic role of and to determine how it functions in tumorigenesis in oral squamous cell carcinoma (OSCC), thus providing additional data to help the guidance of clinical practice. RNA-seq data and relevant clinical information were obtained from TCGA. Bioinformatics means used in this study included differential gene expression analysis, KM survival curve analysis, univariate and multivariate Cox regression analyses, nomogram analysis, ROC curve analysis, methylation level analysis, gene function enrichment analysis, and immune cell infiltration analysis. was significantly higher expressed in OSCC tissue than in normal tissue, and the overall survival of the high expression group was significantly lower than that of the normal group. Univariate and multivariate Cox regression analyses showed that could serve as an independent contributing factor to poor OSCC prognosis. The nomograms and ROC curves supported the results above. Its expression was negatively correlated with DNA methylation sites. Analysis of PPI networking and gene functional enrichment showed that was involved in the functional activities of DNA and RNA and was associated with immune cell infiltration. The high expression of is associated with poor prognosis in OSCC, and could be recognized as a novel independent prognostic factor for OSCC.
紧密连接相关的MARVEL蛋白（TAMP）是一种跨膜蛋白，其成员与细胞间紧密连接和上皮重塑相关。含MARVEL结构域3（）是TAMP的成员之一。作为一种参与双细胞紧密连接组装的新型紧密连接蛋白，在肿瘤学领域受到越来越多的关注。本研究旨在探讨的预后作用，并确定其在口腔鳞状细胞癌（OSCC）肿瘤发生中的作用机制，从而为临床实践提供更多指导数据。从TCGA获取RNA-seq数据和相关临床信息。本研究使用的生物信息学方法包括差异基因表达分析、KM生存曲线分析、单因素和多因素Cox回归分析、列线图分析、ROC曲线分析、甲基化水平分析、基因功能富集分析和免疫细胞浸润分析。在OSCC组织中的表达明显高于正常组织，高表达组的总生存期明显低于正常组。单因素和多因素Cox回归分析表明，可作为OSCC预后不良的独立影响因素。列线图和ROC曲线支持上述结果。其表达与DNA甲基化位点呈负相关。PPI网络和基因功能富集分析表明，参与DNA和RNA的功能活动，并与免疫细胞浸润相关。的高表达与OSCC的不良预后相关，可被视为OSCC一种新的独立预后因素。
