Kimm Melanie A, Kästle Sophia, Stechele Matthias M R, Öcal Elif, Richter Lisa, Ümütlü Muzaffer R, Schinner Regina, Öcal Osman, Salvermoser Lukas, Alunni-Fabbroni Marianna, Seidensticker Max, Goldberg S Nahum, Ricke Jens, Wildgruber Moritz
Department of Radiology, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany.
Core Facility Flow Cytometry, Biomedical Center Munich, Ludwig-Maximilians-Universität München, Planegg-Martinsried, Germany.
Front Oncol. 2022 Oct 24;12:959987. doi: 10.3389/fonc.2022.959987. eCollection 2022.
Local ablative therapies are established treatment modalities in the treatment of early- and intermediate-stage hepatocellular carcinoma (HCC). Systemic effects of local ablation on circulating immune cells may contribute to patients' response. Depending on their activation, myeloid cells are able to trigger HCC progression as well as to support anti-tumor immunity. Certain priming of monocytes may already occur while still in the circulation. By using flow cytometry, we analyzed peripheral blood monocyte cell populations from a prospective clinical trial cohort of 21 HCC patients following interstitial brachytherapy (IBT) or radiofrequency ablation (RFA) and investigated alterations in the composition of monocyte subpopulations and monocytic myeloid-derived suppressor cells (mMDSCs) as well as receptors involved in orchestrating monocyte function. We discovered that mMDSC levels increased following both IBT and RFA in virtually all patients. Furthermore, we identified varying alterations in the level of monocyte subpopulations following radiation compared to RFA. (A) Liquid biopsy liquid biopsy of circulating monocytes in the future may provide information on the inflammatory response towards local ablation as part of an orchestrated immune response.
局部消融疗法是治疗早期和中期肝细胞癌（HCC）的既定治疗方式。局部消融对循环免疫细胞的全身作用可能有助于患者的反应。根据其激活状态，髓样细胞既能触发HCC进展，也能支持抗肿瘤免疫。单核细胞在仍处于循环状态时可能就已经发生了某种程度的启动。通过流式细胞术，我们分析了来自21例HCC患者的前瞻性临床试验队列在接受组织间近距离放疗（IBT）或射频消融（RFA）后的外周血单核细胞群体，并研究了单核细胞亚群和单核细胞来源的髓样抑制细胞（mMDSC）的组成变化以及参与协调单核细胞功能的受体。我们发现，几乎所有患者在接受IBT和RFA后，mMDSC水平均升高。此外，我们发现与RFA相比，放疗后单核细胞亚群水平存在不同的变化。（A）未来对循环单核细胞进行液体活检可能会提供有关作为协调免疫反应一部分的对局部消融的炎症反应的信息。
