Instituto Politécnico Nacional, CICATA Unidad Legaria, Legaria 694, Mexico City 11500, Mexico.
Biosensors (Basel). 2022 Nov 5;12(11):973. doi: 10.3390/bios12110973.
In this paper, we present the results of a non-enzymatic electrochemical glucose biosensor based on TiO. An anatase working electrode was synthesized using the spin coating technique with the polymeric precursor method and dispersed TiO nanoparticles. Through scanning electron microscopy, it was observed that the electrode presented an irregular surface with clusters of nanoparticles. Electrochemical characterization indicated that the response was directly related to the morphology of the electrode. In the presence of glucose, the electrode exhibited adsorption behavior toward the molecules, enabling their recognition. The electrode was tested by employing PBS (phosphate buffer solutions) with varying pH values (from 4 to 9), demonstrating its electrochemical stability, even in the presence of glucose. Amperometric characterization was used to determine that the working region appeared from 0.2 mM to 2 mM, with a sensitivity of 4.46 μAcmmM in PBS pH 7. The obtained results suggest that TiO-based electrodes could be used for the detection of glucose concentration in sweat (0.277-1 mM) and saliva (0.23-1.77 mM).
本文提出了一种基于 TiO 的非酶电化学葡萄糖生物传感器的研究结果。采用旋涂技术和聚合物前驱体法合成了锐钛矿工作电极，并分散了 TiO 纳米粒子。通过扫描电子显微镜观察到，电极表面具有不规则的纳米粒子簇。电化学特性表明，响应与电极的形态直接相关。在葡萄糖存在的情况下，电极对分子表现出吸附行为，从而能够识别它们。该电极通过使用不同 pH 值（从 4 到 9）的 PBS（磷酸盐缓冲溶液）进行测试，证明其具有电化学稳定性，即使在存在葡萄糖的情况下也是如此。安培特性分析表明，工作区出现在 0.2 mM 到 2 mM 之间，在 PBS pH 7 下的灵敏度为 4.46 μAcmmM。所得结果表明，基于 TiO 的电极可用于检测汗液（0.277-1 mM）和唾液（0.23-1.77 mM）中的葡萄糖浓度。
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