School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan, 250022, China.
Mikrochim Acta. 2019 Apr 13;186(5):283. doi: 10.1007/s00604-019-3374-0.
Biofuel cells (BFCs) based on anodic oxidation and cathodic oxygen reduction represent an attractive alternative to self-powered devices. A glucose/oxygen BFC is described for monitoring glucose. It is making use of a piece of paper carrying a glucose oxidase (GOx) based bioanode, and a bilirubin oxidase (BilOx) based biocathode. The performance of the BFC is affected by the generation of HO, a byproduct of enzymatic glucose oxidation. Therefore, the removal of HO is a crucial step in terms of BFC performance and stability. In addition, direct, unambiguous visual read-out is an ideal way to provide quantitative information. The colorimetric readout system described here is based on the consumption of undesired HO and to convert the extent of energy generation into recognizable variations in color. As the HO travels along the hydrophilic channel by capillary action, the formation of red gold nanoparticles from AuCl leads to the appearance of a red bar that provides distance-based information that can be read visually. The multiply readable information (maximum power density of BFC or visible distance) provides further choices for quantification. It also enhances reliability. The self-powered system based on the BFC exhibits excellent performance. Glucose can be determined by this method in the 1 to 50 mM concentration range. Graphical abstract Schematic presentation of a paper-supported biofuel cell equipped with a visual distance readout to display the level of energy generation in biofuel cells, and its application in sensing of glucose.
基于阳极氧化和阴极氧还原的生物燃料电池 (BFC) 为自供电设备提供了一种有吸引力的替代方案。本文介绍了一种用于监测葡萄糖的葡萄糖/氧气 BFC。它利用携带葡萄糖氧化酶 (GOx) 基生物阳极和胆红素氧化酶 (BilOx) 基生物阴极的纸张。BFC 的性能受到 HO 的产生的影响，HO 是酶促葡萄糖氧化的副产物。因此，HO 的去除是 BFC 性能和稳定性的关键步骤。此外，直接、明确的视觉读出是提供定量信息的理想方式。这里描述的比色读出系统基于消耗不需要的 HO，并将能量产生的程度转化为可识别的颜色变化。由于 HO 通过毛细作用沿着亲水通道行进，从 AuCl 中形成的红色金纳米粒子导致出现红色条带，提供基于距离的信息，可通过视觉读取。可多次读取的信息（BFC 的最大功率密度或可见距离）为定量提供了更多选择。它还增强了可靠性。基于 BFC 的自供电系统表现出优异的性能。该方法可在 1 至 50mM 的浓度范围内测定葡萄糖。
