Karimi Abdolmaleki Mahmood, Das Anushree, Khambhati Devang P, Shafiee Ali, Dimas Kayli, Velazquez Carlo Alberto, Davachi Seyed Mohammad, Choubtarash Abardeh Sima
Department of Biology and Chemistry, Texas A&M International University, Laredo, TX 78041, USA.
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221, USA.
Bioengineering (Basel). 2022 Oct 23;9(11):606. doi: 10.3390/bioengineering9110606.
Heparin, usually isolated from porcine intestinal mucosa, is an active pharmaceutical ingredient of great material value. Traditionally, diverse types of commercial resins were employed as an adsorbent for heparin retrieval from biological samples. However, more recent years have encouraged the advent of new cost-effective adsorbents to achieve enhanced heparin retrieval. Inexpensive cationic ammonium-functionalized silica gels, monodispersed with larger surface area, porosity, and higher thermal stability, were chosen to evaluate the heparin recovery yield from porcine intestinal mucosa. We demonstrated that higher positively charged and less bulky quaternary modified silica gel (e.g., QDASi) could adsorb ~28% (14.7 mg g) heparin from the real samples. In addition, we also determined suitable surface conditions for the heparin molecule adsorption by mechanistic studies and optimized different variables, such as pH, temperature, etc., to improve the heparin adsorption. This is going to be the first reported study on the usage of quaternary amine-functionalized silica gel for HEP uptake.
肝素通常从猪肠黏膜中分离得到，是一种具有重要物质价值的活性药物成分。传统上，多种类型的商业树脂被用作从生物样品中回收肝素的吸附剂。然而，近年来出现了新型经济高效的吸附剂，以提高肝素的回收率。选择具有较大表面积、孔隙率和更高热稳定性的廉价阳离子铵功能化硅胶来评估从猪肠黏膜中回收肝素的产率。我们证明，带正电荷且体积较小的季铵改性硅胶（如QDASi）可从实际样品中吸附约28%（14.7 mg/g）的肝素。此外，我们还通过机理研究确定了肝素分子吸附的合适表面条件，并优化了不同变量，如pH、温度等，以提高肝素的吸附量。这将是首次报道关于季铵功能化硅胶用于肝素摄取的研究。
