Hayashi Koichiro, Kishida Ryo, Tsuchiya Akira, Ishikawa Kunio
Department of Biomaterials, Faculty of Dental Science, Kyushu University, Fukuoka 812-8582, Japan.
Bioengineering (Basel). 2022 Oct 31;9(11):627. doi: 10.3390/bioengineering9110627.
Synthetic scaffolds with the ability to prevent fibrous tissue penetration and promote bone augmentation may realize guided bone regeneration without the use of a barrier membrane for dental implantation. Here, we fabricated two types of honeycomb scaffolds of carbonate apatite, a bone mineral analog, whose channel apertures were square (HC-S) and rectangular (HC-R). The side lengths of the HC-Ss and HC-Rs were 265.8 ± 8.9; 817.7 ± 2.4 and 267.1 ± 5.2 μm, respectively. We placed cylindrical HC-Ss and HC-Rs on the rabbit calvaria. At 4 weeks post-implantation, the HC-Ss prevented fibrous tissue penetration from the top face via the channels, which allowed the new bone to reach the top of the scaffold from the bottom face or the calvarium. In contrast, in the HC-Rs, fibrous tissues filled the channels in the top region. At 12 weeks post-implantation, the HC-Ss were partially replaced with new bone. In the top region of the HC-Rs, although new bone had formed, fibrous tissue remained. According to the findings here and in our previous study, the longer side length rather than the shorter side length of a rectangular scaffold channel aperture is the dominant factor that affects fibrous tissue penetration and new bone augmentation. Furthermore, even though channel aperture areas are similar, bone and fibrous tissue ingrowths are different when the aperture shapes are different.
具有防止纤维组织侵入并促进骨增量能力的合成支架，或许可以在不使用屏障膜的情况下实现牙种植体的引导骨再生。在此，我们制备了两种类型的类骨矿物质碳酸磷灰石蜂窝支架，其通道孔径分别为方形（HC-S）和矩形（HC-R）。HC-S和HC-R的边长分别为265.8±8.9；817.7±2.4和267.1±5.2μm。我们将圆柱形的HC-S和HC-R放置在兔颅骨上。植入后4周，HC-S通过通道阻止了纤维组织从顶面侵入，这使得新骨能够从底面或颅骨到达支架顶部。相比之下，在HC-R中，纤维组织填充了顶部区域的通道。植入后12周，HC-S部分被新骨替代。在HC-R的顶部区域，虽然有新骨形成，但仍有纤维组织残留。根据本研究及我们之前研究的结果，矩形支架通道孔径的较长边长而非较短边长是影响纤维组织侵入和新骨增量的主要因素。此外，即使通道孔径面积相似，当孔径形状不同时，骨和纤维组织的向内生长情况也不同。
