Sunpapao Anurag, Suwannarach Nakarin, Kumla Jaturong, Dumhai Reajina, Riangwong Kanamon, Sanguansub Sunisa, Wanchana Samart, Arikit Siwaret
Agricultural Innovation and Management Division (Pest Management), Faculty of Natural Resources, Prince of Songkla University, Songkhla 90110, Thailand.
Research Center of Microbial Diversity and Sustainable Utilization, Chiang Mai University, Chiang Mai 50200, Thailand.
J Fungi (Basel). 2022 Mar 23;8(4):335. doi: 10.3390/jof8040335.
Dirty panicle disease in coconuts () was first observed in the KU-BEDO Coconut BioBank, Nakhon Pathom province, Thailand. The occurrence of the disease covers more than 30% of the total coconut plantation area. The symptoms include small brown to dark brown spots and discoloration of male flowers. Herein, three fungal strains were isolated from infected samples. Based on the morphological characteristics the fungal isolates, they were classified into two genera, namely, (Al01) and (FUO01 and FUP01). DNA sequences of internal transcribed spacer (ITS), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), translation elongation factor 1-α (), and RNA polymerase II second largest subunit () revealed Al01 as , whereas DNA sequences of ITS, , and identified FUO01 and FUP01 as and , respectively. A pathogenicity test by the agar plug method demonstrated that these pathogens cause dirty panicle disease similar to that observed in natural infections. To the best of our knowledge, this is the first report on the novel dirty panicle disease in coconuts in Thailand or elsewhere, demonstrating that it is associated with the plant pathogenic fungi , , and .
椰子脏穗病（）首次在泰国佛统府的KU - BEDO椰子生物库中被发现。该病的发生面积占椰子种植总面积的30%以上。症状包括从棕色到深棕色的小斑点以及雄花变色。在此，从受感染样本中分离出三株真菌菌株。根据真菌分离株的形态特征，它们被分为两个属，即（Al01）和（FUO01和FUP01）。内部转录间隔区（ITS）、甘油醛 - 3 - 磷酸脱氢酶（GAPDH）、翻译延伸因子1 - α（）和RNA聚合酶II第二大亚基（）的DNA序列显示Al01为，而ITS、和的DNA序列分别将FUO01和FUP01鉴定为和。通过琼脂块法进行的致病性试验表明，这些病原体引起的脏穗病与自然感染中观察到的相似。据我们所知，这是泰国或其他地方关于椰子新型脏穗病的首次报道，表明它与植物病原真菌、和有关。
