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LL-37类似物肽对[具体菌种]的体外抗真菌活性

In Vitro Antifungal Activity of LL-37 Analogue Peptides against spp.

作者信息

Pinilla Gladys, Coronado Yenifer Tatiana, Chaves Gabriel, Muñoz Liliana, Navarrete Jeannette, Salazar Luz Mary, Taborda Carlos Pelleschi, Muñoz Julián E

机构信息

Grupo de Investigación REMA, Faculty of Health Sciences, Universidad Colegio Mayor de Cundinamarca, Calle 28 # 5-B-02, Bogotá 110311, Colombia.

Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Carrera 30 # 45-03, Ciudad Universitaria, Bogotá 111321, Colombia.

出版信息

J Fungi (Basel). 2022 Nov 7;8(11):1173. doi: 10.3390/jof8111173.

DOI:10.3390/jof8111173
PMID:36354940
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9694450/
Abstract

Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with that presents resistance to several antifungals. Therefore, generating new alternatives of antifungal therapy is fundamental. One of these possibilities is the use of antimicrobial peptides, such as LL-37, which acts on the disruption of the microorganism membrane and promotes immunomodulatory effects in the host. In this study, we evaluated the in vitro antifungal activity of the LL-37 analogue peptides (AC-1, LL37-1, AC-2, and D) against different spp. and clinical isolates obtained from patients with vulvovaginal candidiasis. Our results suggest that the peptides with the best ranges of MICs were LL37-1 and AC-2 (0.07 µM) against the strains studied. This inhibitory effect was confirmed by analyzing the yeast growth curves that evidenced a significant decrease in the fungal growth after exposure to LL-37 peptides. By the XTT technique we observed a significant reduction in the biofilm formation process when compared to yeasts untreated with the analogue peptides. In conclusion, we suggest that LL-37 analogue peptides may play an important antimicrobial role against spp.

摘要

近几十年来，真菌感染有所增加，导致了相当高的发病率和死亡率，主要发生在免疫抑制患者或入住重症监护病房的患者中。真菌对传统抗真菌治疗的耐药性已成为一个公共卫生问题，尤其是对多种抗真菌药物呈现耐药性的情况。因此，开发新的抗真菌治疗替代方案至关重要。其中一种可能性是使用抗菌肽，如LL-37，它作用于破坏微生物膜并在宿主中发挥免疫调节作用。在本研究中，我们评估了LL-37类似物肽（AC-1、LL37-1、AC-2和D）对不同念珠菌属以及从外阴阴道念珠菌病患者分离出的临床菌株的体外抗真菌活性。我们的结果表明，对所研究菌株而言，最低抑菌浓度（MIC）范围最佳的肽是LL37-1和AC-2（0.07µM）。通过分析酵母生长曲线证实了这种抑制作用，该曲线表明暴露于LL-37肽后真菌生长显著下降。通过XTT技术，我们观察到与未用类似物肽处理的酵母相比，生物膜形成过程显著减少。总之，我们认为LL-37类似物肽可能对念珠菌属发挥重要的抗菌作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d9c6/9694450/3b358dd67367/jof-08-01173-g007.jpg
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