Programa de Pós-graduação em Processos Interativos de Órgãos e Sistemas. Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, BA, 40110-100, Brazil.
Instituto de Tecnologias de Saúde, SENAI/CIMATEC, Salvador, BA, 41650-010, Brazil.
BMC Complement Med Ther. 2021 Nov 24;21(1):286. doi: 10.1186/s12906-021-03445-5.
Oral candidiasis is an opportunistic disease caused by fungi of the Candida genus. The occurrence of Candida spp. resistance to the commercial antifungal drugs points to the search for alternative treatments. Propolis has been successfully used in the treatment of infectious diseases for centuries. It has been proposed that an ultrasound pretreatment in the propolis extraction protocol can enhance the concentrations of molecules with antimicrobial activities in the final extract. Thus, this study aimed to compare the antifungal activity against oral Candida spp. isolates of green and red propolis extracts submitted or not to an ultrasound pretreatment before the extraction procedure.
Candida spp. were isolated from denture stomatitis lesions and identified by sequencing. Oral Candida spp. isolates and reference strains were submitted to broth microdilution assays using commercial antifungals and Brazilian green and red propolis extracts submitted or not to an ultrasound pretreatment. Minimal Inhibitory Concentrations (MIC) and Minimal Fungicide Concentrations (MFC) were determined and biofilm formation interference was evaluated for resistant isolates.
C. albicans, Candida tropicalis and Candida dubliniensis were isolated from denture stomatitis lesions. Growth inhibition was observed in all Candida isolates incubated with all green and red propolis extracts. At lower doses, red propolis extracts presented significant antifungal activity. The ultrasound pretreatment did not promote an increase in the antifungal activity of green or red propolis. Three isolates, which were highly resistant to fluconazole and itraconazole, were susceptible to low doses of red propolis extracts. These same three specimens had their biofilm formation inhibted by red propolis ethanolic extract.
Thus, red propolis can be faced as a promising natural product to be used in the auxiliary antifungal therapy of denture stomatitis.
口腔念珠菌病是一种由念珠菌属真菌引起的机会性疾病。念珠菌属对商业抗真菌药物的耐药性的出现表明需要寻找替代治疗方法。蜂胶已成功用于治疗传染病已有数百年的历史。有人提出，在蜂胶提取方案中进行超声预处理可以提高最终提取物中具有抗菌活性的分子的浓度。因此，本研究旨在比较未经超声预处理和经超声预处理的绿蜂胶和红蜂胶提取物对口腔念珠菌分离株的抗真菌活性。
从义齿性口炎病变中分离出念珠菌属，并通过测序进行鉴定。将口腔念珠菌属分离株和参考株用商业抗真菌药物和未经超声预处理和经超声预处理的巴西绿蜂胶和红蜂胶提取物进行肉汤微量稀释试验。测定最小抑菌浓度（MIC）和最小杀菌浓度（MFC），并评估耐药分离株的生物膜形成干扰。
从义齿性口炎病变中分离出白色念珠菌、热带念珠菌和都柏林念珠菌。用所有绿蜂胶和红蜂胶提取物孵育的所有念珠菌分离株均观察到生长抑制。在较低剂量下，红蜂胶提取物表现出显著的抗真菌活性。超声预处理并没有促进绿蜂胶或红蜂胶的抗真菌活性增加。三株对氟康唑和伊曲康唑高度耐药的分离株对红蜂胶提取物的低剂量敏感。这三个标本的生物膜形成也被红蜂胶乙醇提取物抑制。
因此，红蜂胶可以作为一种有前途的天然产物，用于义齿性口炎的辅助抗真菌治疗。
