Liu Weixiao, Meng Lixia, Zhao Weiling, Wang Zhanchao, Miao Chaohua, Wan Yusong, Jin Wujun
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Metabolites. 2022 Nov 7;12(11):1078. doi: 10.3390/metabo12111078.
Label-free quantitative proteomic (LFQ) and widely targeted metabolomic analyses were applied in the safety evaluation of three genetically modified (GM) maize varieties, BBL, BFL-1, and BFL-2, in addition to their corresponding non-GM parent maize. A total of 76, 40, and 25 differentially expressed proteins (DEPs) were screened out in BBL, BFL-1, and BFL-2, respectively, and their abundance compared was with that in their non-GM parents. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that most of the DEPs participate in biosynthesis of secondary metabolites, biosynthesis of amino acids, and metabolic pathways. Metabolomic analyses revealed 145, 178, and 88 differentially accumulated metabolites (DAMs) in the BBL/ZH58, BFL-1/ZH58, and BFL-2/ZH58×CH72 comparisons, respectively. KEGG pathway enrichment analysis showed that most of the DAMs are involved in biosynthesis of amino acids, and in arginine and proline metabolism. Three co-DEPs and 11 co-DAMs were identified in the seeds of these GM maize lines. The proteomic profiling of seeds showed that the GM maize varieties were not dramatically different from their non-GM control. Similarly, the metabolomic profiling of seeds showed no dramatic changes in the GM/non-GM maize varieties compared with the GM/GM and non-GM/non-GM maize varieties. The genetic background of the transgenic maize was found to have some influence on its proteomic and metabolomic profiles.
除了三种转基因玉米品种BBL、BFL-1和BFL-2各自对应的非转基因亲本玉米外，还采用了无标记定量蛋白质组学（LFQ）和广泛靶向代谢组学分析对这三种转基因玉米品种进行安全性评估。在BBL、BFL-1和BFL-2中分别筛选出76、40和25个差异表达蛋白（DEP），并将它们的丰度与各自非转基因亲本中的丰度进行比较。京都基因与基因组百科全书（KEGG）通路富集分析表明，大多数DEP参与次生代谢物的生物合成、氨基酸的生物合成以及代谢途径。代谢组学分析显示，在BBL/ZH58、BFL-1/ZH58和BFL-2/ZH58×CH72的比较中分别有145、178和88个差异积累代谢物（DAM）。KEGG通路富集分析表明，大多数DAM参与氨基酸的生物合成以及精氨酸和脯氨酸代谢。在这些转基因玉米品系的种子中鉴定出3个共同差异表达蛋白和11个共同差异积累代谢物。种子的蛋白质组分析表明，转基因玉米品种与其非转基因对照没有显著差异。同样，种子的代谢组分析表明，与转基因/转基因和非转基因/非转基因玉米品种相比，转基因/非转基因玉米品种没有显著变化。发现转基因玉米的遗传背景对其蛋白质组和代谢组图谱有一定影响。
