Acute and Subacute Toxicity Studies of Extracts by Oral Administration in Rodents.

The present study aimed to evaluate the acute and subacute toxicity profiles of extracts in mice using the methods described in the guidelines of the OECD. In the acute toxicity study, the LD value was greater than 2000 mg/kg. The subacute toxicity study of extracts showed no significant changes in body or organ weights. The administration of extracts to mice at a dose of 200 mg/kg led to an increase in white blood cells, platelets and hemoglobin. Moreover, the aqueous extract of only decreased liver aspartate aminotransferase (ASAT) levels at a dose of 200 mg/kg, and creatinine and urea levels did not show any significant alterations compared to the control group. Our results showed that the extracts of caused a slight increase in alanine aminotransferase (ALAT) and triglycerides. The histological study showed that mice treated with extracts experienced some histopathological changes in the liver, particularly with the methanolic extract, and slight changes in the kidneys and pancreas. Regarding the renal profile, no toxicity was observed. These results provide basic information on the toxicological profile of used in traditional medicine.